For a long time optical microscopy was held back by a presumed limitation: that it would never obtain a better resolution than half the wavelength of light. Helped by fluorescent molecules the Nobel Laureates in Chemistry 2014 ingeniously circumvented this limitation. Their ground-breakin...
The forms of super-resolution microscopy work in different ways. For example, with PALM and STORM, only a small fraction of the fluorescent markers on molecules are turned on, or photoactivated, at any given moment, allowing them to be localized independently with high precision. Running this pr...
SR-SIM stands for “super-resolution structured illumination microscopy” and is a fluorescence microscopy procedure. Objects are irradiated with laser light. This light excites special fluorescent molecules in the sample so that they re-emit light at a different wavelength. The microscopic image then ...
EDITORIAL Seeing in super-resolution The 2014 Nobel Prize in Chemistry has been awarded in recognition of super-resolution fluorescence microscopy. To fully realize the tremendous promise of these methods for biological discovery, there are still challenges to be overcome. We at Nature Methods need ...
Initially conceived to study biology below the diffraction limit, super-resolution microscopy (SRM) is emerging as a powerful tool for studying synthetic materials owing to its nanometric resolution, multicolour ability and minimal invasiveness. In this Review, we provide an overview of the pioneering ...
Initially conceived to study biology below the diffraction limit, super-resolution microscopy (SRM) is emerging as a powerful tool for studying synthetic materials owing to its nanometric resolution, multicolour ability and minimal invasiveness. In this Review, we provide an overview of the pioneering ...
RS:Scientists use super-resolution microscopy to reveal nanometer-scale details inside cells; the method revolutionized light microscopy and earned some of its inventors the 2014 Nobel Prize in Chemistry. For this paper, an international cohort of AI researchers and microscopists developed an...
Indeed, the impact of super-resolution techniques on research has been such that the 2014 Nobel Prize in Chemistry was awarded to Stefan W. Hell, Eric Betzig, and William E. Moerner; Stefan Hell for the development of STED microscopy, and Eric Betzig and William Moerner for work leading to...
For hundreds of years optical microscopy was relegated to the diffraction-limited regime, unable to resolve details smaller than approximately 200 nm (in XY) and 500 nm (in Z). That limit…
The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micrometer ran...