For a long time optical microscopy was held back by a presumed limitation: that it would never obtain a better resolution than half the wavelength of light. Helped by fluorescent molecules the Nobel Laureates in Chemistry 2014 ingeniously circumvented this limitation. Their ground-breaki...
EDITORIAL Seeing in super-resolution The 2014 Nobel Prize in Chemistry has been awarded in recognition of super-resolution fluorescence microscopy. To fully realize the tremendous promise of these methods for biological discovery, there are still challenges to be overcome. We at Nature Methods need ...
Initially conceived to study biology below the diffraction limit, super-resolution microscopy (SRM) is emerging as a powerful tool for studying synthetic materials owing to its nanometric resolution, multicolour ability and minimal invasiveness. In this Review, we provide an overview of the pioneering ...
The forms of super-resolution microscopy work in different ways. For example, with PALM and STORM, only a small fraction of the fluorescent markers on molecules are turned on, or photoactivated, at any given moment, allowing them to be localized independently with high precision. Running this pr...
Superresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of th
Indeed, the impact of super-resolution techniques on research has been such that the 2014 Nobel Prize in Chemistry was awarded to Stefan W. Hell, Eric Betzig, and William E. Moerner; Stefan Hell for the development of STED microscopy, and Eric Betzig and William Moerner for work leading to...
SR-SIM stands for “super-resolution structured illumination microscopy” and is a fluorescence microscopy procedure. Objects are irradiated with laser light. This light excites special fluorescent molecules in the sample so that they re-emit light at a different wavelength. The microscopic image then...
RS:Scientists use super-resolution microscopy to reveal nanometer-scale details inside cells; the method revolutionized light microscopy and earned some of its inventors the 2014 Nobel Prize in Chemistry. For this paper, an international cohort of AI researchers and microscopists developed a...
super-resolution microscopy (SRM) is emerging as a powerful tool for studying synthetic materials owing to its nanometric resolution, multicolour ability and minimal invasiveness. In this Review, we provide an overview of the pioneering studies that use SRM to visualize materials, highlighting exciting...
The recent 2014 Nobel Prize in chemistry honored an era of discoveries and technical advancements in the field of super-resolution microscopy. However, the applications of diffraction-unlimited imaging in biology have a ...