For a long time optical microscopy was held back by a presumed limitation: that it would never obtain a better resolution than half the wavelength of light. Helped by fluorescent molecules the Nobel Laureates in Chemistry 2014 ingeniously circumvented this limitation. Their ground-breaki...
The 2014 Nobel Prize for Chemistry has gone to Eric Betzig, Stefan Hell and William Moerner for developing super-resolution microscopy techniques based on the fluorescence of molecules. The prize is worth SEK 8m (£690,000) and will be shared by the three winners, who will receive their med...
super‐resolutionA big honor for small objects: The Nobel Prize in Chemistry 2014 was jointly awarded to Eric Betzig, Stefan Hell, and William E. Moerner "for the development of super-resolved fluorescence microscopy". This Highlight describes how the field of super-resolution microscopy developed...
The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micrometer ran...
Although all super-resolution techniques surpass the resolution of traditional light microscopy, some make more gains than others. SIM roughly doubles the resolution, dropping down to about 100 nanometers. PALM and STORM can resolve features on the order of 15 nanometers. STED, says Hell, “can...
Super-resolution microscopy is a technique that can "see" beyond the diffraction of light, providing unprecedented views of cells and their interior structures and organelles. The technique has garnered increasing interest recently, especially since its developers won the Nobel Prize in Chemistry in 20...
SR-SIM stands for “super-resolution structured illumination microscopy” and is a fluorescence microscopy procedure. Objects are irradiated with laser light. This light excites special fluorescent molecules in the sample so that they re-emit light at a different wavelength. The microscopic image then...
The recent 2014 Nobel Prize in chemistry honored an era of discoveries and technical advancements in the field of super-resolution microscopy. However, the applications of diffraction-unlimited imaging in biology have a ...
RS:Scientists use super-resolution microscopy to reveal nanometer-scale details inside cells; the method revolutionized light microscopy and earned some of its inventors the 2014 Nobel Prize in Chemistry. For this paper, an international cohort of AI researchers and microscopists developed ...
How exactly can researchers utilize super resolution microscopy? With microscopy and super resolution, we can see details that we have never been able to see before. Thanks to this technology, the resolution is dramatically improved. Researchers can utilize this information in their scientific research...