Since the time of Janssen and Hooke, optical microscopy has made great impact to human civilization. There are several important parameters in optical microscopy, including contrast, resolution, penetration depth, noninvasiveness, etc. Among them, contrast might be the most important factor....
Single-molecule super-resolution imaging provides a non-invasive method for nanometer-scale imaging and is ideally suited to investigations of quasi-static... Julie,S.,Biteen,... - 《Chemphyschem》 被引量: 137发表: 2012年 Imaging Nanostructures by Single-Molecule Localization Microscopy in Organic...
Editorial — Introduction to the special issue on super-resolution optical microscopy Zhen-Li Huang1 Ming-Qiang Zhu2 1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics (WNLO), Huazhong University of Science and Technology, Wuhan 430074, P. R. China 2Wuhan...
This is very important; one needs to understand what they are dealing with before trying to find what it does. A variety of models, from lipid films to giant plasma membrane vesicles and a range of methods, from physical chemistry to super-resolution microscopy, were used to gain a great ...
How does photoactivated localization microscopy (PALM) work? And what use can PALM microscopy be to you? This short introduction to PALM gives you the answers!Written by: ZEISS Microscopy A Nobel Prize-winning breakthrough Superresolution microscopy has stepped into the limelight earlier this year ...
The early 20th century brought about exciting advancements in optical microscopy, especially with the development of fluorescence microscopy. Initially, fluorescence images lacked clarity, but the addition of a small orifice—the pinhole—in 1950 led to the development of the confocal microscope, which ...
Lecture1,PartOne 1 Grading: 15%:quizbasedonlastlecture 15%:quizbasedonProf.Fink’sclass 70%:finaltermpaperinEnglish 2 3 Outlineoftheclass 1Introductiontophotonsandopticalinstrumentation 2LCSM-LaserConfocalScanningMicroscope 3Super-resolutionmicroscopy1:fluorescence 4Super-resolutionmicroscopy2:microsphereimagi...
Huang, B., Bates, M. and Zhuang, X., “Super-resolution fluorescence microscopy,”Annu Rev Biochem(2009) 78:993–1016. Inoué, S. and Spring, K.R.,Video Microscopy, Second Edition, Plenum Publishing (1997). Lichtman, J.W. and Conchello, J.A., “Fluorescence microscopy,”Nat Meth...
HRTEM (high resolution transmission electron microscopy) Powerful tool for elucidating the real structure. It is of special importance for polygonal quasicrystals, since it reveals the arrangement of columnar clusters in when viewing the structure along its unique n-fold axis. ...
is widely performed, usually with stereo microscopes or macroscopes. Advanced fluorescence techniques have become more and more important in the last decades. The rapid increase in the application of confocal microscopy has changed the perspective in biological investigation from planar to the third dime...