Find out more about Leica super-resolution microscopy solutions and how they can empower you to visualize in fine detail subcellular structures and dynamics.
Self-inspired learning for denoising live-cell super-resolution microscopy SN2N, a Self-inspired Noise2Noise module, offers a versatile solution for volumetric time-lapse super-resolution imaging of live cells. SN2N uses self-supervised data generation and self-constrained learning for training with...
Super-Resolution Microscopy – Get Your Free e-Book for DownloadUntil recently, the diffraction of light had placed a fundamental limit on how far biologists could peer into cells with optical microscopes, preventing them from resolving features less than 250 nm in size, missing critical structure...
Despite the advantages of traditional fluorescence microscopy, the technique is hampered in ultrastructural investigations due to the resolution limit set by the diffraction of light, which restricts the amount of information that can be captured with standard objectives. In the past few years, a ...
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront ...
Super-resolution Microscopy in Plant Cell Imaging. Although the development of super-resolution microscopy methods dates back to 1994, relevant applications in plant cell imaging only started to emerge in 2010. Since then, the principal super-resolution methods, including structured-illu... G Komis,...
Optical super-resolution microscopy in neurobiology Understanding the highly plastic nature of neurons requires the dynamic visualization of their molecular and cellular organization in a native context. How... SJ Sigrist,BL Sabatini - 《Current Opinion in Neurobiology》...
Abstract: 作为捕捉纳米分辨率生物标本图像的主流技术,电子显微镜(electron microscopy, EM)在扫描宽视场(field-of-view, FOV)标本时非常耗时。本文研究了一项具有挑战性的任务——大因子电磁图像超分辨(EM image super-resolution, EMSR),它在缩短扫描时间、放宽采集条件和扩大成像视场(FOV)方面大有可为。通过利用电磁...
Several super-resolution microscopy (SRM) techniques have been devised over the years which can be utilized to overcome diffraction limit. These techniques have opened a new window in biological research. However, SRM methods are highly vulnerable to the lack of appropriate fluorophores and other ...
impeding the observation of instantaneous intracellular dynamics. Here we develop a deep-learning based single-frame super-resolution microscopy (SFSRM) method which utilizes a subpixel edge map and a multicomponent optimization strategy to guide the neural network to reconstruct a super-resolution image...