super-resolution microscopy.In this brief review,we survey recent advances in using deep learning to enhance the performance of biological super-resolution microscopy,focusing primarily on computational reconstruction of super-resolution images.Related key technical challenges are discussed.Despite the ...
We sought to determine whether intracellular staining for IFNγ production in CD19 CAR-T after coculture with myeloma cells could be used as a surrogate assay to test for the presence of CD19lowmyeloma cells instead ofdSTORM microscopy. However, the IFNγ assay only worked in two of the ten...
The size of these labels makes the difference in super-resolution microscopy. Coupled to fluorescent dyes, VHHs are useful tools in super-resolution. As Eckert points out, “A less than 2 nanometer epitope-label displacement minimizes linkage error.” He adds, “The fluorophores of conventional d...
Review Articles Literature References Contributing Authors Despite the advantages of traditional fluorescence microscopy, the technique is hampered in ultrastructural investigations due to the resolution limit set by the diffraction of light, which restricts the amount of information that can be captured ...
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront ...
Three super-resolution platforms are outlined in this chapter鈥攕tructured illumination microscopy, stimulated emission depletion microscopy and localization microscopy. Each method differs in terms of maximum achievable resolution, ease of use, phototoxicity and applicability to 3D and live-cell imaging. ...
While electron microscopy allows identifying cell substructures until a resolution of ∼1 nm, the resolution of fluorescence microscopy is restricted to ∼200 nm due to the diffraction limit of light. However, the advantage of this technique is the possibility to identify and co-localize ...
the resolution of conventional microscopy is restricted by the diffraction limit. This makes it a challenge to observe subcellular processes happened in nanoscale. The development of superresolution microscopy provides a solution to this challenge. Here, we briefly review several commonly used super-reso...
These gaps were enriched with phosphorylated S5 RNA pol II and were sensitive to the cellular transcriptional state. Taken together, we could visualize the decondensed chromatin regions together with active RNA pol II for the first time using super-resolution microscopy....
super-resolution microscopy; SIM; TIRFM; STED; SMLM; PALM; STORM; organelles; membrane contact sites1. Introduction The well-defined images of isolated organelles that many textbooks still display today have been rendered obsolete. The development of high-resolution imaging techniques has allowed ...