Error: No initial signal plan loaded for tls 'nodexx'. Loading of additional-files failed. Quitting (on error). 这可能是因为在写入net文件时没有考虑交叉口是要设置交通灯的,因而将其type选择为无信号灯的priority类型。将其改为trffic_light型就可以正常运行。 另外,当交叉口有固定的多种配时方案时,...
Along with the navigation we have moved to native browser tabs for managing your different open views within Sumo Logic, allowing you to take better advantage of the full features of your browser, and improving performance when loading or switching between different features of the platform. You c...
ALDH þ cells caused tumour growth in all NSG mice tested (3/3), but injection of ALDH À cells failed to grow tumours (0/3) (Fig. 1b). Consistent with CSC characteristics, ALDH þ cells were more resistant to radiation than were ALDH À cells (Fig. 1c). Western blot ...
Our initial attempt to generate PCNA shRNA-knockdown cells stably expressing a shRNA-resistant S2-KR fusion construct failed due to cell growth inhibition a week after transfection. Therefore, we transiently overexpressed a FLAG-tagged S2-KR fusion protein in HEK293T cells (Fig.2b) and looked f...
In addition to GβL, it is possible that one or more additional components of the mTOR and other non-mTOR complex regulators (depicted as question mark [?]) can be modified by SUMO and influence complex assembly and activation. mTOR, mechanistic target of rapamycin; mTORC, mTOR complex; ...
this interaction is insufficient for an efficient SUMO transfer in vivo and needs to be stabilised either by additional E2 interactions or by E3 ligases16. Particularly, E3 ligases interact with the substrate and the charged E2 enzyme to catalyse the discharge of the thioester-bound SUMO from the...