Error: No initial signal plan loaded for tls 'nodexx'. Loading of additional-files failed. Quitting (on error). 这可能是因为在写入net文件时没有考虑交叉口是要设置交通灯的,因而将其type选择为无信号灯的priority类型。将其改为trffic_light型就可以正常运行。 另外,当交叉口有固定的多种配时方案时,...
The new Entity Relationship Graph provides a graphical visualization of all related Entities in an Insight, as well as additional relationships beyond the Insight. The Entity Relationship Graph (and the Related Entities list) displays all Entities involved in the Insight (those referred to in a reco...
due to S2-KR overexpression were also observed at additional CFSs in HEK293T cells (Supplementary Fig.4a), as well as in HeLa and HCT116 cells (Supplementary Fig.4b, c). To further confirm that RNAPIIo is associated with CFS primarily during S-phase, we compared levels of RNAPIIo bound...
showing ALDH, SAE2, Ubc9 and global SUMOylation (SUMO-2,3) level of ALDH þ and ALDH À cells isolated from colorectal cancer cell line HT29 and primary colon cancer PDX tumour tissue (386532 and 354313 correspond to PDX tissues from different patient specimen); GAPDH, loading control....
In addition to GβL, it is possible that one or more additional components of the mTOR and other non-mTOR complex regulators (depicted as question mark [?]) can be modified by SUMO and influence complex assembly and activation. mTOR, mechanistic target of rapamycin; mTORC, mTOR complex; ...
Additional information Competing interests The authors declare that they have no competing interests. Authors’ contributions GHC and SSQ carried out most of the experiments and drafted the manuscript. LXT, LDX, YH and LJX critically revised the experiment design and manuscript. JY, YSL, YSH, SDH,...
While the redistribution of SUMO from one pool of targets to another is unquestionably involved in the SUMO-mediated responses to stress, findings by us and other groups support the need for additional SUMO synthesis as a likely part of the process. First, using a serial dilution approach in ...
additional E2 interactions or by E3 ligases16. Particularly, E3 ligases interact with the substrate and the charged E2 enzyme to catalyse the discharge of the thioester-bound SUMO from the E2 enzyme to the substrate. Although the majority of SUMO E3 ligases identified are nuclear localised, a ...