December 12, 2024 Sumo Logic is enhancing the trial onboarding process with the introduction of pre-loaded sample data, allowing new users to immediately engage with the platform's features without the initial need to provide their own data. This streamlined experience is designed to demonstrate ...
Samples containing SUMO remnant-modified peptides, Lys-C peptides corresponding to complete proteome or tryptic peptides for the LFQ were analysed with a 90, 240 or 150 min linear gradient of acetonitrile in 0.1% formic acid, respectively, at a flow rate of 250 ml per min, and the ...
To reduce sample complexity before tryptic digestion and immunoaffinity purification, we enriched His6- tagged SUMOylated proteins using IMAC. A work flow illustrating the sample preparation, IMAC purification, trypsin digestion and immunoprecipitation is shown in Fig. 1b. Enrichment of SUMOylated proteins...
Sample preparation for mass spectrometry Two-thirds of eluted protein sample from HA Immunoprecipitation were run on a 4-15% Mini-PROTEAN (Bio-Rad) gel to separate proteins and remove synthetic HA peptide. Samples were stained using Silver Stain for Mass Spectrometry Kit (Thermo Scientific, 24600...
prefix = path.basename(options.osm_file).replace('.osm.xml','')ifoptions.prefix: prefix = options.prefix basename = path.join(options.output_directory, prefix) netfile = prefix +'.net.xml'netconvertOpts += vclassRemove[options.vehicle_classes] + ["-o", netfile]# write configcfg = bas...
The corresponding peptide mixtures were analyzed with an Agilent 1100 HPLC system coupled to a LTQ–FT–Ultra mass analyzer (Thermo Scientific) using a Phenomenex 150/3 Kinetex C18 column (2.6 μm particle size, 100 A pore diameter) at 60 °C and a flow rate of 0.2 ml/min. The gradient...
Telegraf requires Go version 1.13 or newer, the Makefile requires GNU make. Install Go>=1.13 (1.15 recommended) Clone the Telegraf repository: cd ~/src git clone https://github.com/influxdata/telegraf.git make cd ~/src/telegraf make
This sample/opt/trident/sensor/conf/trident-sensor.cfgfile specifies the use of four workers. log_file = /opt/trident/sensor/logs/trident-shipper.log input_directory = /opt/trident/sensor/output sensor_name = demo-sensor sensor_id = 99b63729-007v-038u-8t42-51dikn72j7g ...
VPC flow logs Application code trace Security audit events Cluster performance metrics Access records from malicious IPs or users Infrastructure related events Third-party exceptions Five phases of log management Step 1: Instrument and collect Install a collector to collect data from any part of your ...
Of course, in a real situation, only the top level symptom, in this case, either the HTTP 5xx Count or HTTP 4xx Count might be apparent to the on-call user. The troubleshooting challenge is to rapidly isolate the causal chain as illustrated in the flow using Root Cause Explorer. To set...