The enzyme displays phosphatase activity, which is optimal at neutral pH (7.0) and 65 掳C. Substrate screening and kinetic analysis showed AraL to have low specificity and catalytic activity towards several sugar phosphates, which are metabolic intermediates of the glycolytic and pentose phosphate ...
et al. A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity. Am. J. Trop. Med. Hyg. 70, 119–124 (2004). Article CAS Google Scholar Moll, K., Ljungström, I., Perlmann, H., Scherf, A. & Wahlgren, M. (eds) in:Methods in Malaria Research MR4/ATCC...
It has broad substrate specificity, optimal activity between pH 7.0 and 8.0, is dependent on a divalent cation for activity, and is not inhibited by fluoride. It is stimulated more than 10-fold by HPr(Ser(P)) or a mutant derivative of HPr, S46D HPr, in which the regulatory serine is ...
The lysosomal fraction of rat liver has been investigated with the view to determining the possible multiplicity of enzymes exhibiting phosphatase activity. After ammonium sulfate purification, diethylaminoethyl cellulose chromatography resolved the enzyme mixture into a nucleotidase with maximum activity at ...
The activity of succinate, NAD-dependent isocitrate and alcohol dehydrogenases, and of acid phosphatases has been studied cytochemically in wood rays of Betula populifolia Marsh . and Populus tremuloides Michx . in late winter and in spring. The contact cells of the rays which provide the ...
A cellular protein, previously described as p55, binds specifically to the plus strand of the mouse hepatitis virus (MHV) leader RNA, We have purified this protein and determined by partial peptide sequencing that it is polypyrimidine tract-binding protein (PTB) (also known as heterogeneous ...
The present research shows that the plague toxin does not affect the activity of alpha-amylase and glucose-6-phosphatase in the liver of albino mice and rats. In the early stages of plague intoxication the sugar content of the blood in albino rats remains unchanged, and in the later stages ...
Inhibition of APase activity by applying Brefeldin A, an inhibitor of APase production and secretion, significantly suppressed the tuber swelling and moderately affected the stolon elongation and the tuberization frequency. During tuber development, sucrose serves as the main soluble sugar for long-...
BvPECP2L exhibited low activity. RT-PCR experiments for BvPECP1S showed the increased expression in young leaf and root tip under salt-stress whereas the increased expression in all organs under phosphate deficiency. The expression level of BvPECP2L in salt stressed young leaf and root tip was...
S6Pase activity in the fat body remains low during the feeding period, abruptly increases at the wandering and decreases to a low level after gut purge. 20-Hydroxyecdysone (20E) increases S6Pase activity in the fat body of feeding larvae, and the activation is dose-dependent. Cell ...