The distribution of various glucosidase, exo-glucanase and sugar phosphatase activities bore no clear relationship to actual hexose concentrations. However, the distribution of invertase activity was parallel to that of glucose, and sucrose synthetase was most active in regions where fructose and sucrose...
The enzyme displays phosphatase activity, which is optimal at neutral pH (7.0) and 65 掳C. Substrate screening and kinetic analysis showed AraL to have low specificity and catalytic activity towards several sugar phosphates, which are metabolic intermediates of the glycolytic and pentose phosphate ...
et al. Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. J. Biol. Chem. 281, 36149–36161 (2006). Article CAS Google Scholar Hunter, S. et al. InterPro in 2011: new developments in the family and domain prediction database...
Protein phosphatase type 2A (PP2A) activity is required for the sucrose induction of fructan metabolism in wheat leaves, as shown in experiments with the addition of the specific inhibitor okadaic acid (OA) together with sucrose. However, a decrease in total PP2A activity has been found along ...
Suc-phosphate phosphatase; PGI: phosphoglucose isomerase; NI: neutral invertase; SUSY: sucrose synthase; HK: hexokinase; FK: fructokinase; PGM: phosphoglucomutase; UDPase: UDP-glucose pyrophosphorylase; SS: starch synthase; SBE: starch branching enzyme; SDE: starch debranching enzyme; AMY: amylase; ...
When substrates are metabolized, the level of glycolytic intermediates such as fructose 1,6-diphosphate (FBP) rises, which stimulates the oligomeric bifunctional enzyme HPr kinase/HPr-Ser46-P phosphatase (HPrK/P) to generate HPr-Ser46-P, whereas dephosphorylation of HPr-Ser46-P is activated by...
Sucrose phosphate phosphatase SPS: Sucrose phosphate synthase SPT: Sugar phosphate translocator SRA: Sugar Research Australia SSR: Simple sequence repeats or microsatellites SUCEST: Sugarcane expressed sequence tag database SUGIT: Sugarcane Iso-Seq transcriptome database SuSy: Sucrose synthase ...
By upregulating the expression level of the SAUR gene and inhibiting the activity of PP2C. D phosphatase, cell growth is induced through extracellular acidification, cell wall relaxation, and increased phosphorylated H+-ATPases [33, 34]. For the GH3 gene, Zhao et al. [35] found that MsGH...
To quantify the activity of the insulin signalling pathway, the level of AKT phosphorylation (pAkt) was determined by western blotting. For each sample, five adults were homogenised in 150 μL of RIPA buffer with cOmplete protease inhibitor cocktail (Roche) and phosphatase inhibitors (Roche). ...
(from 183 to 188 bp downstream of the start codon) in the ATP-binding domain (Extended Data Fig.2) is expected to disrupt its kinase activity32. MM-CDPK27-CR2 and M82-CDPK27-CR3, with 5-bp deletions (from 184 to 188 bp downstream of ATG), result in the premature termination ...