Laboratory of Biochemistry, University of Amsterdam, Amsterdam (The Netherlands);;elsevierBiochim Biophys ActaScholte H. R. and Tager J. M. (1965) On the nature of the compartmentation of the substrate-linked phosphorylation in rat-liver mitochondria. Biochim. biophys Acta 110, 252-258....
The effects of uncouplers and valinomycin plus nigericin (in the presence of K+) were studied on the apparent Kmfor substrates and apparent Vmaxof the following energy-linked reactions catalyzed by submitochondrial particles: oxidative phosphorylation, NTP-33Piexchange, ATP-driven electron transfer from...
Cyclin/cyclin-dependent kinase (CDK) complexes are critical regulators of cellular proliferation.A complex network of regulatory mechanisms has evolved to control their activity, including activating and inactivating phosphorylation of the catalytic CDK subunit and inhibition through specific regulatory proteins...
As protein phosphorylations are essential components of the messenger systems from these receptors, we have examined whether Gi could be functionally coupled to protein kinases in the activated cell. We have found that Giα2 is directly linked to a serine kinase in Balb/c3T3 fibroblasts, and ...
SENSORS AND ACTUATORS BRho J K, Kang J A, Choi M H, et al. Enhanced kinase sens- ing by radio-phosphorylation and oriented immobilization of a GBP-linked kinase substrate on a gold surface[J]. Sens Actua- tors, A, 2011,160:632-636....
Reagent is succinimidyl moiety linked to kinase substrate; can be linked to free primary or secondary amine in compound to be phosphorylatedUS6335176 1998年10月16日 2002年1月1日 Pharmacopeia, Inc. Incorporation of phosphorylation sitesUS6335176 * Oct 16, 1998 Jan 1, 2002 Pharmacopeia, Inc. ...
Like protein phosphorylation, glycosylation is believed to play signaling and regulatory roles as aberrant glycosylation has been linked to numerous disease states from cancer to diabetes and neurodegeneration. Previous efforts to identify substrates of OGT have largely been based on the enrichment of...
A new strategy for enhanced sensing of kinases was developed using radio-phosphorylation and a polypeptide with an affinity for gold that was linked directly to kinase substrates. Gold-binding polypeptide (GBP)-linked GST-fusion substrates were efficiently immobilized on gold-coated slides, which is ...
Signal transduction from the insulin receptor to downstream effectors is attenuated by phosphorylation at a number of Ser/Thr residues of insulin receptor substrate-1 (IRS-1) resulting in resistance to insulin action, the hallmark of type II diabetes. Ser/Thr residues can also be reversibly ...
The phosphorylation mimic mutants, Amph1 (S293E) and Amph1 (S293D), showed significantly reduced affinity for endophilin, a protein involved in synaptic vesicle endocytosis. Introduction of point mutations in the catalytic domain of CDKL5, which are disease-causing missense mutations found in Rett...