Laboratory of Biochemistry, University of Amsterdam, Amsterdam (The Netherlands);;elsevierBiochim Biophys ActaScholte H. R. and Tager J. M. (1965) On the nature of the compartmentation of the substrate-linked phosphorylation in rat-liver mitochondria. Biochim. biophys Acta 110, 252-258....
Substrate Phosphorylation and Feedback Regulation in:底物磷酸化和反馈调节in,调节,In,and,And,底物磷酸化,底物,磷酸化,磷酸化和,磷酸化与 文档格式: .pdf 文档大小: 1.46M 文档页数: 10页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0 文档热度:
Contacts present in the pre-dephosphorylation complex involve eIF2α residues previously observed to interact with the kinase PKR in a prephosphorylation enzyme/substrate complex29. Notably, the side chain of PKR Phe489 is inserted into the cleft formed by eIF2α Met44 and Tyr81 (a counterpart ...
The effects of uncouplers and valinomycin plus nigericin (in the presence of K+) were studied on the apparent Kmfor substrates and apparent Vmaxof the following energy-linked reactions catalyzed by submitochondrial particles: oxidative phosphorylation, NTP-33Piexchange, ATP-driven electron transfer from...
Activation of hormone-sensitive lipase occurs by phosphorylation of this enzyme in several serine residues. Upon activation of Agrp neurons, we found decreased levels of phosphorylated hormone-sensitive lipase (p-HSL) in WAT compartments (Fig. 4e and Supplementary Figure 6). Together, these ...
Specifically, mitochondria were isolated from the heart and kidney cortex and OM to measure: (i) respiratory rates at different states, (ii) citrate synthase activity, (iii) membrane potential, (iv) state 3 duration of ADP phosphorylation, (v) respiratory control ratio (RCR; state 3/state 2...
Cyclin/cyclin-dependent kinase (CDK) complexes are critical regulators of cellular proliferation.A complex network of regulatory mechanisms has evolved to control their activity, including activating and inactivating phosphorylation of the catalytic CDK subunit and inhibition through specific regulatory proteins...
Phosphatase recognition sites were experimentally determined by measuring dephosphorylation of 6,218 microarrayed Tyr(P) peptides representing confirmed and theoretical phosphorylation motifs from the cellular proteome. A broad continuum of dephosphorylation was observed across the microarrayed peptide substrates...
phosphorylation and its downstream targets, which in turn promotes AKT-driven glycolysis. This pathway generates energy that supports the growth and progression of HCC in the hypoxic tumor microenvironment (Fig.3A) [50]. RBMY (RNA binding motif protein Y-linked), an oncofetal protein, has been...
we identify THEMIS as an uncharacterized substrate for the phosphatase SHP1. Saturated mutagenesis assays and mass spectrometry analysis reveal that phosphorylation of THEMIS at the evolutionally conserved Tyr34 residue is oppositely regulated by SHP1 and the kinase LCK. Similar toTHEMIS−/−mice,TH...