TMB (tetramethylbenzidine) peroxidase substrates are chromogenic substrates used in ELISA applications. In a chromogenic or colorimetric based ELISA, HRP (horseradish peroxidase) is used as a common detection enzyme in the development of immunoassays for diagnostic applications. The unique HRP reaction ...
Polymer materials are used in implants and in many cases polymers mimic the properties of living tissues. Biopolymers are used in the design of scaffolds for regenerative medicine. Similar behavior of cells can be expected on the surfaces of biocompatible polymers and on the surfaces of living ...
A spectroscopic reagent for free chlorine, an enzyme immunochromogenic substrate, a substrate for peroxidase, is used in the ELISA step, which produces a soluble green end product that can be spectral read at 405 nm. This reaction can be terminated with 1% SDS. ...
Hemoglobin was used as the catalytic reagent to catalyse the oxidation of different hydrogen donor substrates viz. 研究了牛血红蛋白(hemoglobin,Hb)作为过氧化物模拟酶,以酸性铬蓝K、罗丹明B、亚甲基蓝不同供氢底物测定H2O2的酶催化反应体系的催化特性和反应条件。 2. We have investigated the effect of ...
applications such as Southern, Northern, and Western blotting. This substrate can also be used in solution-based assays such as immunoassays, DNA probe assays, enzyme assays, and reporter gene assays. Maximum light levels are reached in approximately 10 minutes and glow emission persists for ...
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1.Hemoglobin was used as the catalytic reagent to catalyse the oxidation of different hydrogen donor substrates viz.研究了牛血红蛋白(hemoglobin,Hb)作为过氧化物模拟酶,以酸性铬蓝K、罗丹明B、亚甲基蓝不同供氢底物测定H2O2的酶催化反应体系的催化特性和反应条件。 2.We have investigated the effect of th...
The CDP-Star Substrate (0.4 mM) with Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.
detection in ELISA (ask for comparison of TMB NT-UP66478). The reagent is very stable, at least 18 months under proper storage conditions. Stringent manufacturing conditions ensure excellent lot-to-lot reproducibility. Notes: [a] Incorrect conditions of storage and operating may affect TMB performa...
Different chromogenic substrate mixtures are used with different enzyme labels for developing ELISA. O-phenylenediamine dihydrochloride (OPD) and tetramethylbenzidine (TMB) are commonly employed horseradish peroxidase substrate systems. Tetramethylbenzidine and OPD were compared for sensitivity in Pasteurella ...