1b). As the RBD is the key region for SARS-CoV S-hACE2 recognition, we investigated the binding affinity of hACE2 and S protein though biolayer interferometry (BLI) and enzyme-linked immunosorbent assay (ELISA). The biotin-conjugated hACE2 protein was captured by streptavidin that was ...
Antibodies and reagents The anti-LRP10 (rabbit polyclonal Ab, ThermoFisher, RRID:AB_2555821; 1:200), anti-pTau AT8 (mouse monoclonal Ab, ThermoFisher, RRID:AB_223647; 1:1000), anti-Tau-5 (mouse monoclonal Ab, ThermoFisher, RRID:AB_10980631; 1:1000), anti-LRP1 (mouse monoclonal Ab,...
37], followed by SDS-PAGE to determine levels of LRP10, CD34, NBR1, ACBD3, LRP1, LRP3, LRP6, LDLR, pTau, total tau and β-actin. Levels of Aβ42, Aβ40, APOE and cytokines (IL6, IL10, IL17 and TNFα) were determined using high-sensitive ELISA ...
Reagents Meth was obtained from the National Institutes for Food and Drug Control (Beijing, China), and the following antibodies were used: anti-LC3A/B (12,741 S), anti-Rab7 (9376T), and anti-Rab5 (3547T) antibodies were from Cell Signaling Technology; anti-p62 (ab209012), anti-Stx...
KEY-BIO now has genetic engineering, protein engineering, antibody engineering, marker immunoanalysis and other technical platforms, the use of sufficient technical strength and perfect R & D, production equipment, systematic research and development of bioactive raw materials - intermediates - reagents....
Nevertheless, the SERS signal is influenced by various factors, including the shape, size, and arrangement of metal nanoparticles, and the preparation and operation techniques of the reagents demand specialized spectrometer equipment with higher costs. Despite these challenges, the combination of SERS ...
Quantification of the relative expression of each protein calculated as a ratio to GAPDH levels in different tissues from 2-month-old and 8-month-old mice (n = 10 mice for each group).f, CD38 enzyme activities in different organs during aging were detected by an ELISA (n = 8 ...
The study elucidated the regulatory role of YY1 in the senescence process of beta cells. 2. Materials and methods 2.1. Reagents D-gal (Medchem Express; HY-N0210). Eudesmin (ChemFaces; CFN96266). H2O2 Solution (Sigma; 323381).SA-β-gal Staining Kit (Beyotime; C0602). Cellular Senescence...
the SERS signal is influenced by various factors, including the shape, size, and arrangement of metal nanoparticles, and the preparation and operation techniques of the reagents demand specialized spectrometer equipment with higher costs. Despite these challenges, the combination of SERS and microfluidics...
//www.bdbiosciences.com/en-us/instruments/ research-instruments/research-software/flow- cytometry-acquisition/facsdiva-software CAMAG GE Healthcare Cat# 022.9070 Cat# 17-1154-01 RESOURCE AVAILABILITY Lead Contact Further information and requests for resources and reagents will be fulfilled by the Lead ...