Use1. Peroxidase (horseradish) is one of the most widely used labeling enzymes in ELISA, mainly because of its easy preparation, relatively low price, stable nature, heat resistance and organic solvents, there is little loss of activity upon coupling to the antigen or antibody. It is also com...
Enzymes are protein molecules that catalyze chemical reactions in living organisms. They are involved in many metabolic processes and play a critical role in maintaining the health of the body. In recent years, enzymes have been widely used in disease diagnosis, providing a quick and accurate way ...
An enzyme linked immunosorbent assay (ELISA) using phenolwater extracted lipopolysac-charides as antigen was used for detection and quantitation of antibod... RM Hatfield,B. A. Morris & R. R. Henry - 《Avian Pathology》 被引量: 109发表: 1987年 Enzyme-linked Immunosorbent Assay for Coronavir...
Applications of enzymes in industrial chemical conversions, various forms of catalysis, and more recently in pharmaceutical manufacture are widely known. In medicine, enzymes are used in analytical tests, disease diagnosis, treatment of enzyme deficiencies and wound therapies. In clinical pathology, enzyme...
reacting the selected monoclonal antibody with a blood sample using ELISA to detect serum enzyme ALT or AST which does not bind to an autoantibody, wherein an immunogen used for preparing the monoclonal antibodies is porcine ALT or AST; and the human ALT protein used for selecting a monoclonal...
The expression patterns were analyzed by ΔΔCT method (Livak and Schmittgen 2001) with three replicates, and the expression level of the top part of the cultured medium was used as a calibrator. Results Enzymatic activities in the sawdust medium The mycelia inoculated on the top surface of ...
ELISA methods were used to determine the concentrations of CAI and CAII in spermatozoa, seminal plasma, ovarian follicular fluid, decidual tissues, chorion... SA Akbar,KH Nicolaides,PR Brown - 《Early Human Development》 被引量: 34发表: 1998年 MELK—a conserved kinase: functions, signaling, ca...
The immobilized restriction enzymes could also be used for multiple rounds of digestion; however, longer incubation times were required for successive runs ... Yolanda,Y.,Davidson,... - 《Journal of Separation Science》 被引量: 35发表: 2001年 加载更多研究...
Houston Enzymes is one of the few companies to use xylanase in high amounts, because it also contains enzyme side activities helpful in release of absorbable polyphenols. No other enzyme company comes close to the level of xylanase used in Houston Enzyme products! Increased amounts of lactase, ...
In the first half-reaction, ATP is used to activate the carboxylic acid substrate (1.1), releasing pyrophosphate and forming a reactive acyl-AMP intermediate (1.2) (Fig. 1). Importantly, in the context of inhibitor design, this acyl-AMP intermediate generally remains tightly bound to the ...