Each function is carried out by different parts of the antibody: fragment antigen-binding (Fab fragment) and fragment crystallizable region (Fc region). Fab fragmentis a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy...
The six CDR loops, three on each of the variable domain of the heavy chain (VH) and the light chain (VL), are involved in antigen binding. Therefore, the 3D structure of the antibody determines how it interacts with an antigen and governs its binding properties. Researchers can use the ...
The combining site of the antibody is mostly flat with a protuberance made up of two tyrosines that penetrate the cleft. All six complementarity-determining regions of the Fab contribute at least one residue to the binding; one residue from the framework is also in contact with the lysozyme. ...
The structure shows a CDR loop of the antibody on the GFP scaffold.Yaoguang ChenXiaocheng HuangRongzhi WangShihua WangNing ShiActa Crystallographica Section FCHEN Y, HUANG X, WANG R, et al. The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus [J...
The μ-opioid receptor (μOR) is a G-protein-coupled receptor (GPCR) and the target of most clinically and recreationally used opioids. The induced positive effects of analgesia and euphoria are mediated by μOR signalling through the adenylyl cyclase-inhibiting heterotrimeric G protein Gi. Here...
A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptor T-cell growth factor (TCGF or interleukin-2) is an inducible glycoprotein hormone of molecular weight 15,000 (ref. 1) synthesized and secreted by T lymphoc......
For crystallization, we generated an antibody recognizing the D2R structure (IgG3089) and prepared a novel Fab fragment (Fab3089) of IgG3089. Using the stabilized construct and Fab3089, we successfully obtained crystals of D2R (Supplementary Fig. 4a). The structure of D2R was determined in compl...
Selective Inhibition of Class Switching to IgG and IgE by Recruitment of the HoxC4 and Oct-1 Homeodomain Proteins and Ku70/Ku86 to Newly Identified ATTT ci... Immunoglobulin (Ig) class switching is central to the maturation of the antibody response as IgG, IgA, and IgE are endowed with ...
Antigenic variation among influenza B viruses is different from that of influenza A in several ways. Antigenic shift has not been observed, distinct antigenic variants of influenza B cocirculate, and antigenically similar viruses have been isolated many years apart. To study the mechanism of antigenic...
Polyethylene glycol (PEG) is a polymer routinely used to modify biologics and nanoparticles to prolong blood circulation and reduce immunogenicity of the underlying therapeutic. However, several PEGylated therapeutics induce the development of anti-PEG a