Structure of the PEP core Chloroplasts originated ~1.5 billion years ago when an ancient cyanobacterium was engulfed by a eukaryotic host cell. A typical bacterial RNA polymerase (RNAP) has five core subunits: 2α subunits, β, β', and ω. The RNAP β' subunit is split into two proteins...
This holds promise for a new paradigm shift in biologic therapy via the use of next-generation anti-TNFα agents. Ozoralizumab, a next-generation anti-TNFα agent that was recently approved in Japan, comprises a variable region heavy-chain format. It has a completely different structure from ...
Kritika Chaudhari Basharut A. Syed From the Analyst's Couch17 Nov 2023 Trends in rare disease drug development Rui Chen Sen Liu Shuyang Zhang From the Analyst's Couch06 Nov 2023 The company landscape for artificial intelligence in large-molecule drug discovery Navraj S. Nagra Lieven va...
Classical cadherins mediate cell-cell adhesion through calcium-dependent homophilic interactions and are activated through cleavage of a prosequence in the late Golgi. We present here the first three-dimensional structure of a classical cadherin prosequence, solved by NMR. The prototypic prosequence of...
The design of proteins that bind to a specific site on the surface of a target protein using no information other than the three-dimensional structure of the target remains a challenge1–5. Here we describe a general solution to this problem that starts
Full-length IDOL consists of 445 amino acids, organized in two functional domains: an N-terminal FERM domain and a C-terminal RING domain (Fig. 1A) (12, 14, 28). To study IDOL ligase activity and its structure, recombinant full-length human IDOL protein was purified from insect cells. ...
CD5 is a pan-T cell surface marker that is also present on a subset of B cells, B-1a cells.Functional and developmental subsets of T cells express characteristic CD5 levels that vary over roughly a 30-fold range. Previous investigators have cloned a 1.7
The purpose of this work was to study the gene expression of transient receptor potential (TRP) channels in the mouse. The application of a standardized and quantitative technique, TaqMan RT-PCR, should give information about the pattern and relative importance of TRP channels for murine tissues ...
Here, we show that Agd3 deacetylates GAG in a metal-dependent manner, and is the founding member of carbohydrate esterase family CE18. The active site is formed by four catalytic motifs that are essential for activity. The structure of Agd3 includes an elongated substrate-binding cleft formed...
Using an antibody screen, we identified that in wild-type (WT) and unchallenged brains of mice of both sexes, a subset of microglia coexpresses ionized calcium-binding adaptor molecule 1 (IBA1; encoded by the geneAif1) and the enzyme ARG1. Immunofluorescence analysis of WT mouse brains at ...