Figure 9shows fully automated western blot normalization of HeLa lysates (lanes 2-13), and C2C12 (lanes 14-25), in triplicate. After ERK1 detection (Figure 9a; left), the primary and secondary antibodies were removed with the RePlex reagent mix, to re-stain sa...
活性蛋白整体方案 Active Protein Solutions 免疫印迹(Western Blot)的基本原理,实验步骤及 FAQ 查看 Western blot 实验问题:Western Blot 的常见问题(FAQ) 本文介绍免疫印迹(Western Blot)的基本原理,实验步骤及常见问题(FAQ), 并提供免费 PDF 下载 Western Blot 原理 Western Blot 采用的是聚丙烯酰胺凝胶电泳,被检测...
Petteway Jr., Monitoring plasma processing steps with a sensitive Western blot assay for the detection of the prion protein, J. Virol. Methods 84 (2000) 77-89.Lee DC,Stenland CJ,Hartwell RC,et al.Monitoring Plasma Processing steps with a sensitive Western blot assay for the detection of ...
2B), there was almost no observable effect of the browning agent on the level of UCP1 protein per mg tissue protein in the interscapular brown depot (some 20% increase). The situation regarding the brite/beige depot is dramatically different. Indeed, given the Western blot conditions used, ...
(a) Western blot showing MICAL1 depletion. Loading control: b-tubulin. (b) Snap- shots of time-lapse phase-contrast microscopy movies of cells after control or MICAL1 depletion. Scale bars, 10 mm. (c) Distribution of the abscission times (P ¼ 0.000, non-parametric and distribution-free...
The principle and procedure of the method are similar in all blotting techniques; however, the molecule or particle to be detected might differ. Eastern blot has been slightly modified to form a new form of blotting technique called far-western blotting, which is used to study lipids that are...
c, Determination of HYL1 phosphorylation status. The same blot as that of the upper panel in Fig. 4c with different exposure time. The different forms of HYL1 are as indicated (P+ = phosphorylated HYL1; P− = non-phosphorylated HYL1). The experiment was repeated three times with ...
(E) Western blot detection of recombinant HyNMII motor domain and full-length HyNMII in Hydra lysate as indicated by arrows. The blot was performed using primary antibody solution without (left panel) or with (right panel) 1 mg/mL of the antigenic peptide used for generating the HyNMII anti...
As antibody experts, we are continually amazed by the research and diagnostic opportunities enabled by the specificity of antibodies and antigens.
Southern blot is the process of transfer of DNA fragments that are separated by electrophoresis onto a membrane for immobilization and identification.