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【熟肉】Western Blot常见问题及解决办法-3-非特异性条带 1266 1 10:32 App 【熟肉】新手向实验技术指南,Western Blot Step-by-Step Protocol 3100 -- 6:28 App 【熟肉】Western Blot常见问题及解决办法-4-没有条带 715 -- 3:53 App 【熟肉】爱尔兰国立大学Western Blot教学-2,样品准备 5449 2 6:55...
Western blot 实验心得 step by step 根据自己的实验体会,总结做好 WB 的一些心得,希望对你实验有帮助。围绕着实验的每个步骤展开叙述,这样可能更具体更好理解。 配胶 该部分较为重要的是配制凝胶时要充分混匀,此外应保证试剂的新鲜。常出现的问题有:A. 凝胶出现花纹:此时应检查原料中过硫酸铵(AP)粉末是否受潮。
【讨论帖】Western blot 实验心得 step by step 根据自己的实验体会,总结做好WB的一些心得,与大家分享,不当之处欢迎批评指正。叙述的思路就是围绕着实验的每个步骤展开,这样可能更具体些。 1.配胶: 该部分较为重要的是配制凝胶时要充分混匀,此外应保证试剂的新鲜。 常出现的问题有:A.凝胶出现花纹:此时应检查原...
1、一、 基本原理、分类二、 流程:(自CST网站)三、 流程:(自精编分子生物学实验指南)Western Blot是将蛋白质转移到膜上,然后利用抗体进行检测。对已知表达蛋白,可用相应抗体进行检测。对新基因的表达产物,可通过融合部分的抗体检测Western Blot中文一般称为蛋白质印迹。它是分子生物学、生物化学和免疫遗传学中常用...
Here is a step by step illustration of how to perform a western blot: SDS-PAGE Western Blot Step-by-Step Protocol Western blotting can be used to examine the upregulation of RCAN1, a signaling molecule in neuronal cell types. Materials for Western Blots: ...
Find protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments. Imaging & Analysis Find information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software. ...
WESTERN BLOT PROTOCOL In a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for detection with antibodies. Preparation of Human Cell Extracts (per Yasu Itoh, 5/91) 1. Add 2 volumes of autoclaved H ...
This protocol explains how to conduct a western blot (also known as immunoblot), a technique to detect a target protein among a protein mixture resolved by gel electrophoresis. Steps include sample preparation, gel electrophoresis, membrane blocking, inc
Transfer of high molecular weight proteins using the iBlot 2 Gel Transfer Device wb实验中没有信号条带可能的原因? 1)样本里是否含有目标蛋白;目标蛋白的表达丰度是否在检测范围之内; 2)一抗和二抗是否匹配? 3)一抗和二抗的浓度是否足够? 4)检查抗体是否能够识别样本种鼠中的目标蛋白?