The first step in SOLiD sequencing is preparing the DNA library. Library preparation includes fragmenting DNA or RNA and ligating short adapters to the ends of the fragments. For the SOLiD system, two types of libraries can be created: fragment libraries or mate-paired libraries. The choice dep...
February was an exciting month for Nanopore and it hasn’t ended there. Last week, Clive Brown of Oxford Nanopore Technologies delivered an impressive technical update:GridION X5 – The Sequel.When asked ‘how can I prepare ~1 Mb size DNA?’, Clive advised ‘be gentle’. This question...
P1.32 Hand-held rapid whole genome nanopore sequencing to predict neisseria gonorrhoeae antibiotic susceptibility: steps towards clinic based tailored antimicrobial therapyIntroduction Advances in Point of Care Tests (POCTs), including the capacity to test for multiple Sexually Transmitted Infections (mSTIs...
DNA sequencers still waiting for the nanopore revolution. Pennisi E. Science . 2014Pennisi E. DNA sequencers still waiting for the nanopore revolution. ... BR Jasny,L Roberts 被引量: 19发表: 2003年 The computer revolution in science: steps towards the realization of computer-supported discove...
Viral RNA-dependent RNA polymerases (RdRps) responsible for the replication of single-strand RNA virus genomes exert their function in the context of compl... Supanee,Potisopon,Stéphane,... - 《Nucleic Acids Research》 被引量: 39发表: 2014年 Progress Toward Single-Molecule Nanopore DNA Sequenc...
Evaluation of DNA extraction kits for long-read shotgun metagenomics using Oxford Nanopore sequencing for rapid taxonomic and antimicrobial resistance detection Article Open access 27 November 2024 Development of a portable on-site applicable metagenomic data generation workflow for enhanced pathogen and ...
但是一些基于de Brujin graph的组装软件较为理想的参数还是在60-80×(“Identification of optimum sequencing depth especially for de novo genome assembly of small genomes using next generation sequencing data” 基因组大小评估怎么做? fastp/Trimmomatic/trim_galore ...
"All of the different procedures that will be necessary to maintain the system in production mode need to be thought through ahead of time. You can always bolt it all on later, but it will cost you more money and it won't be native or work as well," Véron said. "In the meantime,...
On the other hand, more modern sequencing (such as Oxford Nanopore) techniques are addressing the limitations of current sequencing methods by extending read lengths to even full-length circRNA sequencing. Longer reads could improve the detection quality and address the variations imposed by circRNA ...
Sović, I.; Križanović, K.; Skala, K.; Šikić, M. Evaluation of hybrid and non-hybrid methods for de novo assembly of nanopore reads.Bioinformatics2016,32, 2582–2589. [Google Scholar] [CrossRef] [PubMed] Jang, M.; Kim, K.; Kim, K. The performance analysis of arm neo...