接下来,为了验证这些肾类器官同样具有肾功能,研究人员将体外培养的类器官移植到小鼠肾内,通过进行标志物荧光染色和渗透实验证明,这些类器官确实能够增加小鼠肾脏血管化并影响肾过滤功能。 图1 Schematic diagram of directed differentiation to gene...
Cell differentiation Stem cells, which can differentiate into fibroblasts,myoblasts, adipocytes, chondrocytes and osteoblasts, have great potential forregenerative medicineand tissue engineering [306,307]. The characteristics of stem cells, such as maintenance ofpluripotencyand proper differentiation, can be ...
Schematic diagram of muscle regeneration. Skeletal muscle tissue regeneration is regulated by a genetic cascade involving Pax7 and MRFs, which drive every step of satellite cell activation, transient expansion of progenitor cells, and the differentiation and formation of new muscle fibers. Interestingly,...
Schematic diagram of differentiation of parthenogenetic stem cells and cardiomyocyte enrichment.Tao YangMichael RubartMark H. SoonpaaMichael DidiéPeter ChristallaWolframHubertus ZimmermannLoren J. Field
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method
Figure 1. HSCs give rise to all the cell lineages of blood. Upon cell division, HSCs can achieve a variety of cell fates, including self-renewal or differentiation into cells with a more restricted differentiation potential. LT-HSCs are the most primitive, with a single cell able to entirely...
We found extensive switching between the A and B compartments during ES cell differentiation, and observed that distinct subsets of genes have concordant A/B compartments status and expression levels. In this regard, these results are similar to what has been seen with nuclear lamina tethering ...
GSEA analysis indicated the differentiated cells were enriched in muscle cell development and myogenesis. Meanwhile, the core genes in each pathway were identified from the GSEA analysis. The in vitro cell research revealed that actin cytoskeleton and myoD were upregulated after 4-week differentiation....
DNA methylation analysis did suggest that our iPSCs more closely resembled their cell-of-origin (umbilical cord-derived MSCs), rather than dermal fibroblasts. However, there were very few PE-specific methylation marks which were preserved through reprogramming and differentiation, and none that explaine...
where they find their way to other tissue and organ sites. (h) If the growth conditions here are favorable, the cancer stem cells start to self-renew and produce more heterogeneous cancer cell mixtures through partial differentiation and switching back to epithelial-like cancer cells. These are ...