Passaging of colonies was done roughly every five days using Gentle Cell Dissociation Reagent (GCDR) from STEMCELL Technologies (Cat No. 100–0485). Stem cell pluripotency was validated using the STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies, Cat No. 05230) and human stem cell ...
We suggest that glucose utilization and lactate production are cell quality attributes that should be considered during the characterization of insulin-producing cells derived from stem cells. Our findings also revealed a gradual metabolic shift from glycolysis, during the initial four stages of ...
Contextual dissection of YAP and TAZ (both as individual proteins and comparing them as paralogs) is key, because prior literature has shown many conflicting roles for these proteins in cell fate changes, both in self-renewal vs. differentiation and survival vs. cell death [21], whereas other ...
To confirm that the expression of secretory cell markers post development is ATOH1-dependent, we analyzed Atoh1-creER-T2/flox;RosaTdtomato mice that do not express Atoh1 upon treatment with tamoxifen (see STAR Methods). As expected, tamoxifen administration led to an almost 100% loss of ...
The effects of human amniotic fluid stem cell (hAFSC) transplantation on bladder function and molecular changes in spinal cord-injured (SCI) rats were investigated. Four groups were studied: sham and SCI plus phosphate-buffered saline (SCI + PBS), human embryonic kidney 293 (HEK293) cells...
A small, but growing, number of experiments have sought to evaluate the impact of adipose-derived cells in large animal disease models; however, these studies in the dog, goat, horse, pig, rabbit, or sheep have been hampered by the lack of appropriate monoclonal antibody reagents for cell ...
(Fig.4a). Interestingly, long-term stem cell markers were enriched in the C2; Mk progenitor markers12,37were largely enriched in C3; and Mk lineage markers12,37were enriched in C3 and C4 (Fig.4c). Trajectory and pseudotime analysis showed that C2 cells reside at the top of haematopoiesis...
aSchematic of experimental workflow. EPSC: expanded potential stem cells, iNSC: induced neural stem cells, eNSC: endogenous neural stem cells, mGIC: mouse glioblastoma initiating cells.bTwo dimensional principal component analysis of cell types, representing normalized RNA sequencing data of eNSC, iN...