Cell Type Pluripotent Stem Cells Species Human Application Cell Culture, Expansion, Maintenance Brand TeSR Area of Interest Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology Formulation Category Serum-Free View More Data Figures Figure 1. mTeSR™ Plus Maintains Optimal...
Cell Type Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells Species Human Application Cell Culture, Differentiation, Toxicity Assay Brand STEMdiff Area of Interest Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology ...
Stem cell cul- ture was done using a commercially available media kit (mTeSR Plus; Cat No. 100–0276, STEMCELL Technolo- gies) following the manufacturer's published protocol [29]. Briefly, cell colonies were grown on Matrigel-coated 6-well plates (Matrigel: Cat No. 354277, Corning; Plates...
源叶 santa 博迈德 麦克林 亚科因 陶术 Solarbio 普利莱 DSHB Proteintech Elabscience Serumwerk Bernburg AG Biogems Peprotech Progen Polyplus Qiagen 全式金 polysciences Rockland Roche罗氏 Sigma Selleck ScienCell Stressmarq SouthernBiotech SurModics Sangon Biotech STEMCELL Technologies TBD TAKARA The Well Vector...
Cell-cell interactions are required for development and homeostasis in multicellular organisms from insects to mammals. A critical process governed by these interactions is cell competition, which fu...
hESC cell culture WA01 (H1) hESCs were obtained from WiCell at passage 46 and expanded to generate a master bank within the first 5 passages. hESCs were cultured on Matrigel (Corning, cat. no. 354277) in mTeSR1 or mTeSR Plus (STEMCELL Technologies, cat. no. 85850 and 100-0276). Pass...
#100–0276) media, and refreshed daily. mTeSRTMPlus media contained 0.5 mM sodium butyrate until Day 11. Reprogramming efficiency was calculated at Day 22, and was derived from dividing the resultant number of colonies as visualised on alkaline phosphatase (AP) staining by the starting cell numbe...
One of the challenges in Good Manufacturing Practice (GMP)-compliant human induced pluripotent stem cell (hiPSC) production is the validation of quality control (QC) tests specific for hiPSCs, which are required for GMP batch release. This study presents a comprehensive description of the validation...
To assess the differentiation potential of patient-derived iPSC lines into cells from the three germ layers, three-dimensional iPSC aggregates known as embryoid bodies (EBs) were specifically induced to differentiate into cell types characteristic of each layer. iPSC were cultured to 80% confluence ...
• mTeSR™ Plus (Catalog #100-0276) • TeSR™-E8™ (Catalog #05990) Subtype Specialized Media Cell Type Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells Species Human Application Cell Culture, Differentiation Brand STEMdiff Area of Interest Stem Cell Biology Formulati...