Passaging of colonies was done roughly every five days using Gentle Cell Dissociation Reagent (GCDR) from STEMCELL Technologies (Cat No. 100–0485). Stem cell pluripotency was validated using the STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies, Cat No. 05230) and human stem cell ...
16 (Xinhua) -- The Abu Dhabi Stem Cells Center (ADSCC) has introduced a new approach to diabetes management using umbilical cord-derived mesenchymal stem cell (MSC) therapy, the United Arab Emirates state news agency WAM reported on Thursday. Mesenchymal stem cells have the ability to self-...
Several protocols have been developed to make stem cell-derived insulin-producing cells. However, there is a lack of knowledge regarding the bioprocess parameters associated with these differentiation protocols and how they can be utilized to increase the cell yield. Methods We investigated various ...
To confirm that the expression of secretory cell markers post development is ATOH1-dependent, we analyzed Atoh1-creER-T2/flox;RosaTdtomato mice that do not express Atoh1 upon treatment with tamoxifen (see STAR Methods). As expected, tamoxifen administration led to an almost 100% loss of ...
we hypothesized that the Microniche system could also expand functional Mk stem and/or progenitor cells. Microniche-based culture of mouse HSCs resulted in significantly higher proportions and absolute cell numbers of mouse phenotype-defined long-term HSCs (LT-HSCs) as well as Mk-biased HSCs compa...
A small, but growing, number of experiments have sought to evaluate the impact of adipose-derived cells in large animal disease models; however, these studies in the dog, goat, horse, pig, rabbit, or sheep have been hampered by the lack of appropriate monoclonal antibody reagents for cell ...
The effects of human amniotic fluid stem cell (hAFSC) transplantation on bladder function and molecular changes in spinal cord-injured (SCI) rats were investigated. Four groups were studied: sham and SCI plus phosphate-buffered saline (SCI + PBS), human embryonic kidney 293 (HEK293) cells...
Hippo effectors YAP and TAZ control cell fate and survival through various mechanisms, including transcriptional regulation of key genes. However, much of
Here we develop a strategy to compare the epigenetic and transcriptional make-up of primary GBM cells (GIC) with patient-matched expanded potential stem cell (EPSC)-derived NSC (iNSC). Using a comparative analysis of the transcriptome of syngeneic GIC/iNSC pairs, we identify a glycosaminoglycan...
were passaged once a week using Gentle Cell Dissociation reagent (StemCell Technologies, Vancouver, BC, Canada, 100-0485) according to the manufacturer’s protocol. Cells were split 1:10–1:50 to a new well for further maintenance. The iPSCs were cultured in an environment of 37 °C, 5%...