羊抗鼠平滑肌22α(Smooth muscle 22 alpha, SM22α)单克隆抗体购买自美国Cell Signaling Technology(CST)公司。SDS-PAGE电泳设备及转膜仪、光学显微镜、压缩雾化吸入器、酶标仪等购自上海生工公司。RT-PCR、cDNA试剂盒购自大连宝生物工程有限公司)...
每次10min。1:10000加入二抗（Jackson，货号115-035-003），室温孵育1.5h，1xPBST洗三次，每次10min，曝光拍照。SOCS3一抗来源于 Proteintech，货号66797-1-Ig，1xPBST 1:3000稀释使用。STAT3一抗来源于Cell signaling technology，货号9139S，1xPBST 1:1000稀释使用。结果 1、蓝光照射后细胞形态变化 ...
SCC 大小按肿瘤 TNM 分级标准 ,分为Tl 、T2 、 13 和 T4 四级 、 50 例患 者中 Tl-T2 级 27 例(C组 ),T3-T4 级 23 例( D 组) ,所有标本均通过 HE染色确诊 、,1.2 试剂p-STAT3 ( 兔来源多克隆抗体) 和 p-Akt(兔来源 多克隆抗体 ) 研究试剂购 自美 国 Cellsignalingtechnology,p-ERKl/2...
β-actin一抗、STAT3一抗、p-STAT3一抗、羊抗兔二抗均购自美国Cell Signaling Technology公司。 2.THP-1细胞培养和分化:THP-1单核细胞培养在含有10%胎牛血清、1%双抗、1%非必需氨基酸的RPMI1640培养基中,于37℃、5%CO2的培养箱中孵育培养;在细胞处于对数生长期且状态良好时加入浓度为100ng/ml的PMA诱导48h,THP...
(Cell Signaling Technology, 9532), MCL-1 (Cell Signaling Technology, 94296), and pSTAT3 (Cell Signaling Technology, 9145) and monoclonal antibodies against E-cadherin (BD Biosciences, San Jose, CA, USA, 564186), Twist1 (Abcam, Cambridge, UK, ab50887) and STAT3 (Cell Signaling Technology, ...
(#19245, 1:2000) were purchased from Cell Signaling Technology (Beverly, MA, USA). Primary antibodies against IL-1β (sc-12742, 1:2000), CD34 (sc-7324, 1:2000), VCAM-1 (sc-13160, 1:2000), and EPAS-1 (sc-13596, 1:2000) were purchased from Santa Cruz Biotechnology (Santa Cruz,...
(all from Sigma Aldrich); SNAIL1, SNAIL2, STAT3, pSTAT3, p65 (Cell Signaling Technology, Danvers, MA, USA); E-cadherin and P-cadherin (BD Bioscience, San Jose, CA, USA); S100A11 (R&D Systems, Minneapolis, MN, USA); TWIST1 (Abcam Cambridge, MA, USA); ZEB2 (in-house made18). ...
(Ser 152/156), MARCKS, survivin, XIAP, cyclin D1, p21 and β-actin were purchased from Cell Signaling Technology Inc (Danvers, MA, USA). The anti-FLAG antibody was purchased from Sigma (St Louis, MO, USA). For western blotting, precasted Nupage electrophoresis gels were purchased from ...
The following antibodies were from Cell Signaling Technology: phospho-Akt (Ser473), Akt, phospho-mTOR (Ser2481), mTOR, Raptor, Rictor, phosphor-STAT3 (Tyr705) and phosphor-STAT3 (Ser727). All secondary antibodies were obtained from Jackson ImmunoResearch Laboratories. The S727D STAT3 mutant was...
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