rsem-calculate-expression \--paired-end \ #双端测序--no-bam-output \ #不输出bam结果(bam是一种文件格式,sam->bam)--alignments-p16-q./04STAR_out/sampleAligned.toTranscriptome.out.bam \#比对所用的线程和输入文件./05rsem_index/Brapa_genome \#索引文件所在目录及其前缀./06rsem_out/sample_rsem...
STAR+RSEM 先比对再进行定量 # build index 建立索引; STAR --runThreadN 10 --runMode genomeGenerate \ --genomeDir arab_STAR_genome \ --genomeFastaFiles 00ref/TAIR10_Chr.all.fasta \ --sjdbGTFfile 00ref/Araport11_GFF3_genes_transposons.201606.gtf \ --sjdbOverhang 149 # 一般为read...
STAR+RSEM 先比对再进行定量 # build index 建立索引;STAR--runThreadN10--runMode genomeGenerate \--genomeDir arab_STAR_genome \--genomeFastaFiles00ref/TAIR10_Chr.all.fasta \--sjdbGTFfile00ref/Araport11_GFF3_genes_transposons.201606.gtf \--genomeSAindexNbases12\--sjdbOverhang149# 一般为reads...
Description of the bug When nextflow is started from an NFS mount (AWS EFS volume), the star_rsem aligner fails at the rsem-calculate-expression step. It appears that this step launches STAR and requires a file system supporting FIFO (na...
RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control. nf-co.re/rnaseq Resources Readme License MIT license Code of conduct Code of conduct Citation Cite this repository Activity Custom properties Stars 0 stars Watc...
RNA seq STAR与rsem的区别 目录 第一课 课程介绍 第二课 RNAseq技术 第三课 RNAseq发展历史 第四课 关于基因的概念 第一课 课程介绍 RNAseq捕获了细胞某一状态下所有的转录信息 DNA是静态信息、RNA是动态信息,是某一时刻DNA表达谱的快照 课程大纲
Subdivided starPathsRSEMT labelingA reverse edge magic total (REMT) labeling for the graph Γ = ( V (Γ), E (Γ)) along with cardinality of p = | V (G)| and q = | E (G)| respectively. A one-one map π : V (Γ) ∪ E (Γ) → {1, 2,,| V (Γ)|+| E (Γ)|} ...
而且STAR众多的输出文件可以满足不同的需求。HISAT2因为索引的优势,可以相对轻松比对跨区域的reads(可变剪切),而Tophat2耗时久,STAR耗内存,HISAT2克服了两个的缺点[2]。 (四)定量软件 介绍的定量软件有RSEM、featureCounts 和HTSeq-count。RSEM像是一个集成的软件,对新手甚是友好,不仅提供了定量的命令,甚至连构建...
RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control. - bioinfo-fiend/rnaseq
RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control. nf-co.re/rnaseq Topics workflow rna-seq pipeline nextflow rna nf-core Resources Readme License MIT license Code of conduct Code of conduct Citation Cite this repo...