# --quantMode TranscriptomeSAM will output alignments translated into tran-script coordinates,为了使用RSEM 进行定量分析做准备;# STAR align 复杂版本 样本R2 STAR --runThreadN 12 --genomeDir arab_STAR_genome \ --readFilesCommand zcat \ --readFilesIn 02clean_data/sample2_1_paired_clean_R1....
STAR+RSEM 先比对再进行定量 # build index 建立索引;STAR--runThreadN10--runMode genomeGenerate \--genomeDir arab_STAR_genome \--genomeFastaFiles00ref/TAIR10_Chr.all.fasta \--sjdbGTFfile00ref/Araport11_GFF3_genes_transposons.201606.gtf \--genomeSAindexNbases12\--sjdbOverhang149# 一般为reads...
rsem-calculate-expression \--paired-end \ #双端测序--no-bam-output \ #不输出bam结果(bam是一种文件格式,sam->bam)--alignments-p16-q./04STAR_out/sampleAligned.toTranscriptome.out.bam \#比对所用的线程和输入文件./05rsem_index/Brapa_genome \#索引文件所在目录及其前缀./06rsem_out/sample_rsem...
Description of the bug When nextflow is started from an NFS mount (AWS EFS volume), the star_rsem aligner fails at the rsem-calculate-expression step. It appears that this step launches STAR and requires a file system supporting FIFO (na...
RNA seq STAR与rsem的区别 目录 第一课 课程介绍 第二课 RNAseq技术 第三课 RNAseq发展历史 第四课 关于基因的概念 第一课 课程介绍 RNAseq捕获了细胞某一状态下所有的转录信息 DNA是静态信息、RNA是动态信息,是某一时刻DNA表达谱的快照 课程大纲
Is it star_rsem using another version of STAR? How could be run with rsem but not with salmon? Author fjosefdz commented May 23, 2024 This is the code I use for running the nfcore rnaseq pipeline throught slurm: #!/bin/bash #SBATCH --job-name=nfcore_rnaseq #SBATCH --partition ...
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RNA seq STAR与rsem的区别 目录第一课 课程介绍第二课 RNAseq技术第三课 RNAseq发展历史第四课 关于基因的概念 第一课 课程介绍RNAseq捕获了细胞某一状态下所有的转录信息DNA是静态信息、RNA是动态信息,是某一时刻DNA表达谱的快照 课程大纲RNAseq的应用第二课 RNAseq技术转录组概念中心法则(高通量测序验证了中...
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