samtools view sample2_Aligned.sortedByCoord.out.bam |head 转录组数据计算表达定量; # rsem prepare reference 从参考基因组中提取原始准备文件 rsem-prepare-reference --gtf 00ref/Araport11_GFF3_genes_transposons.201606.gtf \00ref/TAIR10_Chr.all.fasta \ arab_RSEM/arab_rsem #sample rsem-cal...
测试rsem是否安装 2.png #samtools 查看bam 文件;(每个基因上有多少个reads 的统计信息的bam文件;)#为什么tab文件中后面有三个文件;从原始的read信息到比对文件的过程;samtools view sample2_Aligned.sortedByCoord.out.bam|head 转录组数据计算表达定量;# rsem prepare reference 从参考基因组中提取原始准备文件rsem...
五、RSEM定量 RSEM定量时,同样需要先构建RSEM可以识别的索引文件,之后才可以进行定量 1.安装 conda install rsem 2.构建索引 rsem-prepare-reference \--gtf00ref/Brapa.genome.gtf \#参考基因组的注释文件00ref/Brapa.genome.fasta \#参考基因组05rsem_index/Brapa_genome #索引文件存储位置 ...
RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control. nf-co.re/rnaseq Resources Readme License MIT license Citation Cite this repository Activity Stars 0 stars Watchers 0 watching Forks 0 forks Report repository ...
RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control. nf-co.re/rnaseq Topics workflow rna-seq pipeline nextflow rna nf-core Resources Readme License MIT license Code of conduct Code of conduct Citation Cite this repo...
RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control. - nf-core/rnaseq