c: SPP1+ macrophage在Tumor中的比例显著升高; d: TCGA-COAD数据集中,SPP1+ macrophage在tumor中的比例也显著高; e: 高浸润SPP1+ macrophage的病人,预后差; f: 用于鉴定9个细胞亚型的marker的小提琴图; g: 用流式分选SPP1+ macrophage,验证在tumor中显著高; ...
2024年2月,广西医科大学附属肿瘤医院的向邦德教授团队发表一篇题为“Integrating single‑cell and bulk RNA sequencing reveals CK19 + cancer stem cells and their specific SPP1 + tumor‑associated macrophage niche in HBV‑related hepatocellular carcinoma”的研究成果。本研究通过整合单细胞和Bulk RNA测序数...
g: 用流式分选SPP1+ macrophage,验证在tumor中显著高; h: RNA velocity分析表明,SPP1+ macrophage有可能起源于THBS1+ macrophage; i/j/k/l: 用pySCENIC分析,10个细胞亚型的top 转录因子和调节子,发现STAT1 是SPP1+ macrophage特异性表达; FAP+ fibroblasts 和 SPP1+ macrophage相关性分析 主图 a: 通过scRNA...
2024年2月,广西医科大学附属肿瘤医院的向邦德教授团队发表一篇题为“Integrating single‑cell and bulk RNA sequencing reveals CK19 + cancer stem cells and their specific SPP1 + tumor‑associated macrophage niche in HBV‑related hepatocellular carcinoma”的研究成果。本研究通过整合单细胞和Bulk RNA测序数...
Overall, these results suggest that, despite their complexity, TMEs coordinate coherentresponses that control human cancers and for which CS macrophage polarity is a relevant yet simple variable.Tumor microenvironments (TMEs) areecosystemscomposedofdiversestromal,immune, and cancer cells. The interac-...
In conclusion, we propose anSPP1+macrophage model in CRC, highlighting such macrophages as a promising therapeutic target due to their malignancy markers. 展开 关键词: KeywordsColorectal cancerImmunotherapySingle-cell RNA sequencingSpatial transcriptomicsSPP1+macrophages DOI: 10.1016/j.gendis.2024.101340...
SPP1与多种肿瘤免疫细胞(macrophage、neutrophil、Dendriticcell)浸润呈正相关(P均<005)。在Cox回归生存分析中,我们发现 Bcell,Dendriticcell和SPP1与肺腺癌生存相关(P均<005)。结论 SPP1在非小细胞肺癌中高表达,SPP1与非小细胞肺癌(特别是肺腺癌)患者预后存在紧密的联系,并且可能参与非小细胞肺癌的肿瘤免疫浸润。
Macrophage polarizationPD-L1SPP1TAMsTumor-associated macrophages (TAMs) polarization represents a key regulatory process of tumor progression. However, the underlying mechanisms are unclear. This study aimed to investigate the relationship between secreted phosphoprotein 1 (SPP1) and TAMs in lung ...
Colorectal cancer (CRC) is among the most common malignancies with limited treatments other than surgery. The tumor microenvironment (TME) profiling enables the discovery of potential therapeutic targets. Here, we profile 54,103 cells from tumor and adja
demonstrated that high SPP1 expression on TAMs predicted a poor prognosis in lung adenocarcinoma patients. Additionally, we investigated the expression mechanisms related to SPP1 using human-monocyte-derived macrophages and revealed that the SPP1 expression level increased in macrophage differentiation ...