在这篇Bio-protocol文章中,来自中国科学院上海植物逆境生物学研究中心Alberto Macho研究组的研究人员以农杆菌对本氏烟进行瞬时转化为例,为我们带来分裂荧光素酶互补成像(Split-LUC imaging)的试验方法。 亮点 1. 分裂荧光素酶互补成像是一种比较简单、快速的蛋白质互作体内检测方法; 2. 分裂荧光素酶互补成像不但可以...
这篇高分文章中研究人员使用Molecular Devices SpectraMax i3x多功能微孔板读板机进行Split-LUC测定，验证了SWEET11 和 SWEET 12 与SnRK2的潜在相互作用，从分子层面揭示了干旱胁迫下植物根冠比的调控机制。植物蛋白质相互作用研究方法包括体内、体外及生物信息学（in silico）等。体内研究方法包括酵母双杂交系统（Y2H）...
2、从-80℃冰箱中取出相应的感受态,置于冰上解冻,并做好标记; 3、取2μl质粒加至100μl感受态中,冰浴30min; 4、42℃热激90s,再冰浴5min; 5、加入900μl无抗的LB液体培养基,220rpm震荡培养1h; 6、6000rpm离心5min,仅留100ul上清,并混匀菌体沉淀; 7、混匀后的菌液加至对应抗性的LB平板上,用涂棒...
BosmansLaboratoryJeroenLaboratoryM.LaboratoryBrielsLaboratoryDr.LaboratoryLech-GustavLaboratoryingentaconnectAngewandte ChemieBosmans RPG, Briels JM, Milroy LG, de Greef TFA, Merkx M, Brunsveld L. 2016. Supramolecular control over Split-Luciferase complementation. Angewandte Chemie 55:02807. DOI: https:/...
GL2-LUC siRNA (control siRNA) N/A BiP siRNA N/A GRP94 siRNA N/A ON-TARGET plus Human P4HB (PDI) SMART pool GE Dharmacon Catalog # L-003690-00-0005 ON-TARGET plus Human CALR (CRT) SMART pool GE Dharmacon Catalog # L-0008197-00-0005 Hs_SERPINH1 (HSP47). 6 FlexiTube siRNA QIAGE...
Jean-Luc Guionnet、Richard Cuvillier、Martin Hackett、Antoine Rousseau、David Bausseron、Nicolas Chachignot、Bruno Cheynier、Claude Colpaert、Pierre Cretel、Vincent Debaets、Antoine Defoort、Martin Granger、Raphael Guegnaud、Patrick Guionnet、Franck Lambert、Jacques Leclercq、Philippe Lenglet、Stéphane ...
For Instance, the accumulation level of the NRLuc-GID1 protein is lower than that of the NRLuc-H2A protein (Figure 2A). Figure 2 The interaction of histone-2A and histone-2B was detected by complemented luciferase activity in rice protoplasts. (A) Accumulations of split luciferase fused to...
The product Tweeter allows users to post short messages limited to 50 characters each. Sometimes, users get excited and write messages longer than 50 characters. Instead of rejecting these messages, we would like to add a new feature that will split the message into parts and send multiple mess...
The game is designed by Luc Remond and Romaric Galonnier with illustration by Maud Chalmel. It is for 2-8 players and plays in 15 mins. Overview: In Splito players are working to score points on either side of them with the other players at the table. Each player has two scoring zon...
Half-elf Luc, the agency’s top security expert, has his own secret: he’s responsible for a curse laid from an old assignment. To heal them, he’ll need to perform his job duties with unrelenting excellence and earn time off from his tyrannical boss. When Elle saves Luc’s life, the...