U2AF complex A heterodimeric protein complex consisting of U2 small nuclear RNA auxiliary factor 1 (U2AF1) and U2AF2. U2AF2 and U2AF1 bind to the polypyrimidine tract and AG dinucleotide of the 3′ splice site to
C. Molecular genetic analysis of the heterodimeric splicing factor U2AF: the RS domain on either the large or small Drosophila subunit is dispensable in vivo. Genes Dev. 12, 1010–1021 (1998). Article CAS PubMed PubMed Central Google Scholar Kan, J. L. & Green, M. R. Pre-mRNA ...
The first one is about mutations affecting directly a splicing factor, which impairs specific or general splicing reactions. Often, such mutation is lethal, illustrating the dependence of cells for splicing. Indeed, splicing factors U2AF35 or SF3b subunit 4 have been shown to be essential during...
The protein factor U2AF is an essential component required for pre-mRNA splicing. Mutations identified in the S. pombe large U2AF subunit were used to engineer transgenic Drosophila carrying temperature-sensitive U2AF large subunit alleles. Mutant recombinant U2AF heterodimers showed reduced polypyrimidin...
Splicing factor 3b, subunit 1 SRSF2: Serine/arginine-rich splicing factor 2 U2AF1: U2 small nuclear RNA auxiliary factor 1 BCL-2: B cell lymphoma 2 IAP: Inhibitor of apoptosis BIM: Bcl-2-like protein 11 SRSF1: Serine/arginine-rich splicing factor 1 mTORC1: Mammalian target of...
Interestingly, in Caenorhabditis elegans, METT10 (the ortholog of mouse METTL16) could deposit m6A modification on the 3’-splice site (AG) of the SAM synthetase pre-mRNA to prevent the recognition by the essential splicing factor U2 small nuclear RNA auxiliary factor 35 (U2AF35) [16]. In...
Zuo P, Maniatis T: The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing. Genes Dev. 1996, 10: 1356-1368. 10.1101/gad.10.11.1356. PubMed CAS Google Scholar Kan JL, Green MR: Pre-mRNA splicing of IgM exons M1 and M2 is ...
A. et al. Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. Mol. Cell 23, 49–59 (2006). Article CAS PubMed PubMed Central Google Scholar Lin, P. C. & Xu, R. M. Structure and assembly of the SF3a splicing factor complex of U2...
However, for splicing to occur in some organisms, it is imperative that the spliceosome is assembled on the pre-mRNA. The first step of this process involves the U1 snRNP binding to the 5’ss, splicing factor 1 (SF1) binding to the BPS, and the U2 auxiliary factor (U2AF) binding to...
A. et al. Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. Mol. Cell 23, 49–59 (2006). Article CAS PubMed PubMed Central Google Scholar Lin, P. C. & Xu, R. M. Structure and assembly of the SF3a splicing factor complex of U2...