世联博研公司现货供应abbexa品牌订货号为Catalogue No: abx142288的Splicing Factor U2AF 35 kDa Subunit (U2AF1) Antibody产品
U2 auxiliary factorEarly in the assembly of the spliceosome of eukaryotes the branch-point binding protein (BBP, also called SF1) recognizes the branch point sequence, whereas the heterodimer U2AF consisting of a 65 and a 35 kDa subunit, contacts the polypyrimidine tract and the AG splice site...
The first one is about mutations affecting directly a splicing factor, which impairs specific or general splicing reactions. Often, such mutation is lethal, illustrating the dependence of cells for splicing. Indeed, splicing factors U2AF35 or SF3b subunit 4 have been shown to be essential during...
we assumed that a PS2 pre-mRNA-binding factor, which leads to skipping of exon 5 of the PS2 transcript, exists in nuclear extracts from the hypoxic SK-N-SH cells. To detect the PS2 pre-mRNA-binding factor, we performed a pre-mRNA-binding assay by ultraviolet (UV) crosslinking to variou...
Interestingly, in Caenorhabditis elegans, METT10 (the ortholog of mouse METTL16) could deposit m6A modification on the 3’-splice site (AG) of the SAM synthetase pre-mRNA to prevent the recognition by the essential splicing factor U2 small nuclear RNA auxiliary factor 35 (U2AF35) [16]. In...
(AG) of the SAM synthetase pre-mRNA to prevent the recognition by the essential splicing factor U2 small nuclear RNA auxiliary factor 35 (U2AF35) [16]. In addition, germ cell-specific knockout of mouseMettl16generated byVasa-Cre (induces recombination in germ cells as early as embryonic ...
To test if the identified anchor proteins can also be used to recruit other spliceosomal proteins, we tested the anchoring of U2AF35. U2AF35 interacts with U2AF65 to form the essential U2AF splicing factor complex. U2AF35 interacts with the AG dinucleotide of the 3′-splice site47, and ...
In addition to the coupling of SR proteins to enhancer elements, SR proteins interact with U1 snRNP and the 35 kDa subunit of the heterodimeric factor, U2AF. The second subunit of U2AF, U2AF65, binds SF1 and the pyrimidine tract simultaneously, on the basis of the arginine/serine (RS)-...
It remains unclear what mechanism drives alternative exon regulation by MBNL proteins bound to individual RNAcis-elements. Two previous works proposed involvement of MBNLs in the spliceosome formationviainteracting with U2 auxiliary factor 65 kDa subunit (U2AF65) responsible for 3′ splice site defi...
U2AF is heterodimeric factor composed of 35 kDa and 65 kDa subunits (encoded by U2AF1 and U2AF2 genes respectively). The U2AF65 subunit through its RNA recognition domains binds to the pyrimidine tract of the pre-mRNA, whereas the U2AF35 subunit contacts the 3′-splice site AG nucleotides...