Moreover, the levels of specific Nt‐Abs to SARS‐CoV‐2 Spike protein are correlated to the anti‐S1/S2 IgG titre (R‐squared = 0.47; p < 0.001). CONCLUSIONS. The levels of specific Nt‐Abs to SARS‐CoV‐2 Spike protein are correlated to the anti‐S1/S2 IgG titre, although Nt‐...
Immunological strategies against spike protein: Neutralizing antibodies and vaccine development for COVIDdoi:10.1002/ctm2.184Jiansheng HuangDepartment of Medicine Vanderbilt University Medical Center Nashville TennesseeHui HuangDepartment of Biochemistry and Center of Structural Biology Vanderbilt University Nashville...
SARS-CoV-2 spike (S) protein is a key target for eliciting neutralizing antibodies.aSchematic structures of SARS-CoV-2 virion and its S protein. M, membrane; E, envelope; N, nucleocapsid. Viral RNA is located inside the virion. NTD, N-terminal domain; RBD, receptor-binding domain; FP, ...
Human IgG1-Fc protein “103Cys/Ser” and Cholera toxin subunit B-Alexa555 (CTXB) were from Sino Biological and Molecular Probes, respectively. Antibodies Anti-vimentin antibodies used were: anti-vimentin antibody V9 (sc-6260, unconjugated and Alexa-488-conjugate) from Santa Cruz Biotechnology; ...
Most human monoclonal antibodies (mAbs) neutralizing SARS-CoV-2 recognize the spike (S) protein receptor-binding domain and block virus interactions with the cellular receptor angiotensin-converting enzyme 2. We describe a panel of human mAbs binding to diverse epitopes on the N-terminal domain (NT...
We found that the spike-protein of hCoV-EMC (EMC-S) is incorporated into lentiviral particles and mediates transduction of human cell lines derived from different organs, including lung, kidney and colon, as well as primary human macrophages. Expression of known coronavirus receptors, ACE2, CD13...
A SARS-CoV-2 Antibody Broadly Neutralizes SARS-related Coronaviruses and Variants by Coordinated Recognition of a Virus Vulnerable Site Potent neutralizing SARS-CoV-2 antibodies often target the spike protein receptor-binding site (RBS), but the variability of RBS epitopes hampers broad neu... T ...
The study also analyses the impact of key mutations on interactions with angiotensin converting enzyme 2 (ACE2) and panel of neutralizing monoclonal antibodies. Using 1,44,605 spike protein sequences, global prevalence of mutations in spike protein was observed. The study suggests that SARS-CoV-2...
and a biosafety level 3 laboratory. Here, we report a SARS-CoV-2 surrogate virus neutralization test that detects total immunodominant neutralizing antibodies targeting the viral spike (S) protein receptor-binding domain in an isotype- and species-independent manner. Our simple and rapid test is ...
Serological tests play an essential role in monitoring and combating the COVID-19 pandemic. Recombinant spike protein (S protein), especially the S1 protein, is one of the major reagents used for serological tests. However, the high cost of S protein pro