Positive Predictive Value of SARS-CoV-2 Spike Protein Antibody Test after COVID-19 Vaccination Policiesdoi:10.4103/ijpvm.ijpvm_290_21COVID-19 testingCOVID-19 vaccinesVACCINATION policiesSARS-CoV-2PROTEINSPathum SookaromdeeWiwanitkit, Viroj
Here we present results from a multiplex serology test for assessing the antibody responses to COVID-19. In an initial large cohort, this test shows greater than 99% agreement with COVID-19 PCR test. In a second outpatient cohort consisting of adults and children in Colorado, the IgG ...
incubated with either anti-mouse HRP secondary antibody (Cat: G-21040, Thermo Fisher Scientific) or anti-rabbit HRP secondary antibody (Cat: A16035, Thermo Fisher Scientific). Secondary antibodies were prepared by 1:10,000 dilution in TBS-T. Probed membranes were developed with the Amersham ECL...
Its broad portfolio of oncology product candidates includes individualized and off-the-shelf mRNA-based therapies, innovative chimeric antigen receptor (CAR) T cells, several protein-based therapeutics, including bispecific immune checkpoint modulators, targeted cancer antibodies and antibody-drug...
The rORF2 protein can directly interact with the viral RNA-dependent RNA polymerase, and mutation of rORF2 completely abolishes virus infection, whereas ectopic expression of rORF2 rescues the mutant virus. Furthermore, we show that several rORFs in the −RNA of severe acute respiratory syndrome...
In the seropositive population, seroconversion was defined as a fourfold increase in the antibody levels in comparison to baseline. Neutralizing antibodies were assessed using two methods: a surrogate virus neutralization test (sVNT) and a conventional virus neutralization test (cVNT). The details are...
trachomatis were determined as inclusion forming units (IFU) by counting chlamydial inclusions formed in HEp-2 cells using fluorescein isothiocyanate (FITC)-conjugated monoclonal anti-Chlamydia antibody specific to Chlamydia lipopolysaccharide (LPS) (Denka Seiken Co. Ltd., Tokyo, Japan) [21]. The ...
Many therapeutic antibodies (Abs) and mRNA vaccines, both targeting SARS-CoV-2 spike protein (S-protein), have been developed and approved in order to combat the ongoing COVID-19 pandemic. In consideration of these developments, a common concern has been the potential for Ab-dependent enhancemen...
Hence, we adapted the SPR assay to measure antibody binding and affinity for the native spike protein based on the sera of four SARS-CoV-2-positive individuals in each of the four age groups; we also determined affinity for the B.1.351 variant spike protein for the individuals aged 18–49...
Equal amounts of protein lysate were denatured and subject to SDS-PAGE. Samples were then transferred to a PVDF membrane and probed with the indicated primary antibody (Supplementary Table 2) overnight at 4°C in 5% BSA. The membranes were washed and incubated with the appropriate HRP-...