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Light-activated opsins can trigger intracellular transduction pathways to gen- erate electrical responses in visual photoreception, while light-activated gene transcription can trigger growth responses such as plant photomorphogenesis. In animals, light-induced changes in mRNA levels have been studied in ...
Two plant pathogenic fungi,Bipolaris oryzaeandMagnaporthe oryzae, available in our laboratory were used in the investigation. Both the fungal cultures were routinely maintained in potato dextrose agar (PDA) medium, incubated at 28˚C and stored at 4˚C. Three different concentrations of EOs (1%...
Plant cell growth and development depend about continuous cell proliferation which is restricted to small regions of the flower called meristems. are examined. (Xie et al., 1995) was a first clue as to which mechanism could be used by geminiviruses to induce a permissive cellular state. This...
RNA extraction, reverse transcription PCR (RT-PCR), and northern blot Total RNA was extracted from patients' keratinocytes cultured in vitro using Trizol (Life Technologies), according to the manufacturer's recommendations. cDNA was generated from total RNA by reverse transcription using random hexamers...
Genomic DNAs of hESCs or HAP1 cells were isolated using Gentra Puregene Cell Kit (QIAGEN) per manufacturer protocol. Long-range PCRs in Figures 3B, 3D, 6E, S3B, and S5B were all done using Q5 DNA Polymerase (NEB). Products were resolved on 1% agarose gel stained by SYBR Safe (Invitr...
Average DCp was calculated from 4 independent experiments with 3 individual plants (3 leaves/plant). Data are expressed as fold induction of each point as compared to the wild type. RNA isolation from Arabidopsis seeds imbibed for 16 h was performed using a protocol adapted f...
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The second involves Mlo, a widely conserved plant gene that negatively regulates plant defence responses and might be regarded as a powdery mildew susceptibility gene11,12. Recessive muta- tions in Mlo confer durable resistance to all races of Bgh. Such mutations were originally discovered in ...