salt (EDTA-Na2) and sodium chloride (NaCl) is used as extraction buffer solution, hexadecyl trimethyl ammonium bromide (CTAB) is used as a detergent, 1 mol/L guanidinium isothiocyanate is used as a strong denaturant for inhibiting the activity of RNase, and thus high-quality RNA is ...
RNA extraction was performed on the brain biopsies using the Qiagen FFPE RNA Kit, which includes a DNAse step. RNA from 12 of these MS brain biopsy specimens passed quality control and all have now been sequenced on the Illumina HiSeq2500 platform in two separate runs. Ten of the 12 sequen...
海口誉汇实验器材有限公司 动物组织总RNA提取试剂盒 1 天根 / 按行业标准提供服务 DP431 1449.0 总价最低原则 海口誉汇实验器材有限公司 EP管 5 biosharp BS-100-M,10ml圆底连盖离心管 / 按行业标准提供服务 biosharp 30.0 总价最低原则 海口誉汇实验器材有限公司 离心管泡沫水漂 10 国产 / 按行业标准提供服...
DNA/RNA extraction DNA was obtained from peripheral blood lymphocytes by a simple salting out procedure.14 RNA extraction was per- formed from fresh blood lysates using the Promega (Madison, WI, USA) Total RNA extraction kit according to the manu- facturer's protocol. PTT assay The APC coding...
B cells from NMOSD patients and healthy controls were isolated from PBMCs by magnetic-activated cell sorting techniques according to the protocol provided by Human B-cell Isolation kit II (130-091-151, Miltenyi Biotec, Auburn, CA) and were used for RNA extraction. RNA was reverse transcribed ...
wide range of accessories including various jar and ball sizes, adapter racks for single use vials and tubes, grinding tool materials, CryoKit efficient cell disruption of max. 240 ml cell suspension for DNA/RNA- and protein extraction
A summary of the pros, cons, and methods for DNA and RNA extraction from FFPE tissue for clinical diagnostic and research purposes. Julianne Davis, MSc Exosome-Based Liquid Biopsies: The Potential & the Road Ahead Exosome-Based Liquid Biopsies: The Potential & the Road Ahead ...
RNA extraction and real-time PCR Colon tissues were flushed with PBS and homogenized in TRIzolreagent (Invitrogen, USA) for RNA isolation. Reverse transcription was performed using SuperScript III reverse transcriptase (Invitrogen, USA). Real-time PCR was carried out using a 7500 real-time PCR sys...
For reverse transcription real-time quantitative–PCR(RQ–PCR), RNA was extracted from FFPE samples using the RecoverAll Total Nucleic Acid Extraction kit (Ambion Ltd., Cambridgeshire, UK) and transcribed into complementary DNA (cDNA) using the High Capacity cDNA kit (Applied Biosystems, Warrington...
Within 6 h of collecting peripheral venous blood, as described above, RNA was extracted using the standard TRIzol method then changed to DNA by reverse transcription using the RevertAid First Strand cDNA Synthesis Kit (Thermo, Waltham, MA, USA) according to the manufacturer's instructions. ...