View Price and Availability EURM019 Single stranded RNA (ssRNA) fragments of SARS-CoV-2 View Price and Availability Sigma-Aldrich STRN250 Spectrum™ Plant Total RNA Kit View Price and Availability Sigma-Aldrich EC300 GenElute™-E Single Spin Tissue DNA Kit View Price and Availability ...
Low temperatures cause widespread alterations in the physiology and development of plants. Plants use a variety of regulatory mechanisms to respond to low-temperature conditions and to prepare for freezing temperatures through the process of cold acclimation1,2. Chloroplasts are essential for plant produc...
3.6. Transcript Abundance Analysis RNA was isolated from 10–15 frozen seedlings in Precellys® 24 (Peqlab, Erlangen, Germany) using the RNeasy Plant Mini Kit. The purity of the RNA was determined spectrophotometrically from the A260/A280 ratio. cDNA was synthesized with the High-Capacity ...
Purification of miRNA and other, smaller ncRNA molecules is more challenging due to their short lengths. It is important to select an appropriate RNA isolation method that retains small RNA and avoid using a total RNA purification kit unless the manufacturer specifically states that total...