1. A method of inserting a polynucleotide sequence into a genome of a human cell, comprising: introducing into the human cell: a circular nucleic acid comprising the polynucleotide sequence flanked by a phiC31 first recombination site and a Bxb1 first recombination site; a phiC31 integrase; and...
With draft genomes of several CHO cell lines recently being made available8,9, it is now possible to efficiently engineer the genomic sequence of CHO cells with engineered nucleases. Customized nucleases such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), ...
Cell type-specific gene expression patterns are outputs of transcriptional gene regulatory networks (GRNs) that connect transcription factors and signaling proteins to target genes. Single-cell technologies such as single cell RNA-sequencing (scRNA-seq) and single cell Assay for Transposase-Accessible Ch...
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In order to investigate the genomic context of lung cancer-specific DNA methylation, we analyzed one hypomethylation feature associated with cell of origin (lineage-specific transcription factor binding sites) and one associated specifically with transformation (global hypomethylation). For the TFBS ...
Cell surfa... Randy,Q.,Cron - 《Immunologic Research》 被引量: 88发表: 2003年 Early growth response-1 is required for CD154 transcription. We identified a species-conserved purine-rich sequence located adjacent to the CD154 transcriptional promoter proximal NFAT site, which binds early growth ...
Oct-2 and OBF-1 (also called OCA-B or Bob-1) are B cell?specific transcription factors that bind to the conserved octamer site of immunoglobulin promoters,... Schubart,Karin,Massa,... - 《Nature Immunology》 被引量: 138发表: 2001年 The BOB.1/OBF.1 co-activator is essential for octam...
Transgenes introduced into cancer cell lines serve as powerful tools for identification of genes involved in cancer. However, the random nature of genomic integration site of a transgene highly influences the fidelity, reliability and level of its expression. In order to alleviate this bottleneck, we...
Mapping cell type-specific gene expression quantitative trait loci (ct-eQTLs) is a powerful way to investigate the genetic basis of complex traits. A popular method for ct-eQTL mapping is to assess the interaction between the genotype of a genetic locus
a, In vitro selection scheme consisting of incubation, capture, wash, elution, amplification and transcription steps. The RNA substrate (blue) contains an unpaired adenosine (red, A) and is connected to the RNA library via the single-stranded loop (black). The library contains 40 random nucleot...