Given the remarkable advances in protein nanopore sequencing technology, there is still a strong enthusiasm in exploring alternative nanopore-sequencing techniques, particularly those based on a solid-state nanopore using a semiconductor material. Since solid-state nanopores provide superior material ...
solid-state nanoporesThe solid-state nanopore has attracted much attention as a next-generation DNA sequencing tool or a single-molecule biosensor platform with its high sensitivity of biomolecule detection. The platform has advantages of processability, robustness of the device, and flexibility in the...
The solid-state nanopore-based DNA sequencing technology is becoming more and more attractive for its brand new future in gene detection field. The challenges that need to be addressed are diverse: the effective methods to detect base-specific signatures, the control of the nanopore???s size and...
The ultimate thinness of the 2D nanopores prepared with this method makes them an optimal choice for achieving single-nucleobase resolution to realize solid-state nanopore sequencing. Surface modification and functionalization The chemical modification of a wall surface is an effective method for ...
Nanopore-based sensing is alabel-free techniqueand requires no amplification steps which is for example beneficial when determining an exact viral load in a blood sample or parsing through a sample made up of proteins. ForDNA and RNA sequencing, there are already nanopore-based commercial appl...
Nanopore applicationsAs the key components of nanopore-based nucleic acid sequencing systems, nanopores have drawn more and more scientific interests over these years.Although most of the early nanopore-based sequencers adopted biological nanopores, solid-state nanopores have been gradually growing in ...
lid-State Nanopores SDS-Assisted Protein Transport Through Solid-State NanoporesSDS-Assisted Protein Transport Through Solid-State NanoporesUsing nanopores for single-molecule sequencing of proteins - similar to nanopore-based sequencing of DNA - faces multiple challenges, including unfolding of the complex...
DNA sequencing methods based on nanopores could potentially represent a low-cost and high-throughput pathway to practical genomics, by replacing current sequencing methods based on synthesis that are limited in speed and cost. The success of nanopore sequencing techniques requires the solution to two ...
The feasibility of this approach is demonstrated by characterizing a nanopore-based DNA sequencing device. In the demonstration calculations, the dependence of the device performance on the nucleotide sequence, pore diameter, and applied voltage was determined. Extension of the approach for describing ...
Previously, experiments have been restricted to naturally occurring nanopores, but advances in technology now allow artificial solid-state nanopores to be fabricated in insulating membranes. By monitoring ion currents and forces as molecules pass through a solidstate nanopore, it is possible to ...