SNAP‐tag is a 20kDa versatile protein tag that can be linked to any nucleotide sequence. When fused to a protein, the SNAP‐tag acts as a molecular linker to an array of molecules such as fluorescence probes, biotin, and synthetic compo... 黄维,冯欣欣 被引量: 0发表: 2020年 加载更多站...
The coding sequence of SNAP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. SNAP-tag protein (MW: 19,694) was expressed and purified according to the instructions in the IMPACT™ kit manual (NEB #E6901). The purified SNAP-tag protein was dialyzed into 1X phosphate ...
For the first test of the system, HEK-293T cells were transfected with the DD-SNAP-tag plasmid and with the target sequence in a CMV-driven vector. The target used was the enhanced CyanFluorescent Protein(eCFP W66X) containing an UAG PTC, similar to the eGFPW58X codon described in the ...
This protocol includes DNA sequences encoding scFV–SNAP-tag fusion proteins and outlines strategies for expression, purification and testing of the resulting scFv–SNAP-tag–based immuno-conjugates. All experiments can be performed by a graduate-level researcher with basic molecular biology skills ...
This protocol includes DNA sequences encoding scFV–SNAP-tag fusion proteins and outlines strategies for expression, purification and testing of the resulting scFv–SNAP-tag–based immuno-conjugates. All experiments can be performed by a graduate-level researcher with basic molecular biology skills ...
Each 250 bp DNA template contains a 123 bp 601 nucleosome positioning sequence (gray) [1], a unique 22 bp DNA-barcode (white; 4 barcodes total), and a 5’ biotin-TEG. The 5’ and 3’ linkers (blue) are compatible with cleavage by pAG-MNase (EpiCypher 14-1048, 15-1016) during ...
Each 250 bp DNA template contains a 123 bp 601 nucleosome positioning sequence (gray) [1], a unique 22 bp DNA-barcode (white; 4 barcodes total), and a 5’ biotin-TEG. The 5’ and 3’ linkers (blue) are compatible with cleavage by pAG-MNase (EpiCypher 14-1048, 15-1016) during ...
GPR56 can be activated by collagen III, its endogenous ligand, and by a synthetic seven amino-acid peptide (TYFAVLM; P7) contained within the GPR56 Stachel sequence. However, the mechanisms regulating GPR56 trafficking dynamics and agonist activities are not yet clear. Methods: Here, we ...
CUT&RUN was performed as described in Figure 4. Sequence reads were aligned to 18,793 annotated transcription start sites (TSSs ± 2 kbp). Signal enrichment was sorted from highest to lowest (top to bottom) relative to the H3K4me3 – 500k cells reaction (all gene rows aligned). High, medi...
The K-MetStat Panel controls for 15 widely studied methyl states (see Description) and an unmodified control (me0), with each of the 16 octamers wrapped with two uniquely barcoded DNA templates (A and B). Each 250 bp DNA template contains a 123 bp 601 nucleosome positioning sequence (gra...