For the first test of the system, HEK-293T cells were transfected with the DD-SNAP-tag plasmid and with the target sequence in a CMV-driven vector. The target used was the enhanced CyanFluorescent Protein(eCFP W66X) containing an UAG PTC, similar to the eGFPW58X codon described in the ...
In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates. The coding sequence of SNAP-tag was cloned into a pTX...
Sequence-specific alkylation of DNA, Selective assembly of proteins on DNA nanostructures, Recognition-driven reaction of SNAP-tag, Kinetci control of the SNAP-tag reactionRecognition-driven modification has been emerging as a novel approach to modifying biomolecular targets of interest site-specifically ...
This protocol includes DNA sequences encoding scFV–SNAP-tag fusion proteins and outlines strategies for expression, purification and testing of the resulting scFv–SNAP-tag–based immuno-conjugates. All experiments can be performed by a graduate-level researcher with basic molecular biology skills ...
Each 250 bp DNA template contains a 123 bp 601 nucleosome positioning sequence (gray) [1], a unique 22 bp DNA-barcode (white; 4 barcodes total), and a 5’ biotin-TEG. The 5’ and 3’ linkers (blue) are compatible with cleavage by pAG-MNase (EpiCypher 14-1048, 15-1016) during ...
The description that follows includes systems, methods, techniques, instruction sequences, and computing machine program products that embody illustrative examples of the disclosure. In the following description, for the purposes of explanation, numerous specific details are set forth in order to provide...
(A and B). Each 250 bp DNA template contains a 123 bp 601 nucleosome positioning sequence (gray) [1], a unique 22 bp DNA-barcode (white; 4 barcodes total), and a 5’ biotin-TEG. The 5’ and 3’ linkers (blue) are compatible with cleavage by pAG-MNase (EpiCypher14-1048,15-...
where a particular image included in the message image payload406depicts an animal (e.g., a lion), a tag value may be included within the message tag420that is indicative of the relevant animal. Tag values may be generated manually, based on user input, or may be automatically generated ...
Ubuntu is an open source software operating system that runs from the desktop, to the cloud, to all your internet connected things.
Ready-to-use high expression level SNAP29 cDNA clones are full sequence confirmed with various fusion tags, 1 in lentiviral vector, 13 in expression vector, 1 in cloning vector also available.