b, Bar graph showing the percentage of trans contacts in mitotic cells with eight distinct SMC protein complex configurations. Data are presented as mean values +/- SEM (n = 2 and 3 biological replicates
Recombinant Structural Maintenance Of Chromosomes Protein 3 (SMC3)BMH; BAM; CSPG6; HCAP; SMC3L1; Chondroitin Sulfate Proteoglycan 6; Bamacan; Basement membrane-associated chondroitin proteoglycan; Chromosome-associated peptide [ PROPERTIES ]Residues: Ser994~Glu1181 (Accession # Q9UQE7),with...
We find that TrmBL2, a nucleoid-associated protein that forms a stiff nucleoprotein filament, stalls theSMC complex and establishes a boundary at the site-specific recombination site. TrmBL2 stalls the SMC complex at tens of additional non-boundary loci with lower efficiency. Intriguingly, the ...
2.3 SMC 的系统进化分析 如表 2 所示,东方蜜蜂微孢子虫,海伦脑炎微 孢子虫,颗粒病微孢子虫,康氏泰罗汉孢虫,菲尼斯 毕罗酵母,指间毛廯菌,紫色毛廯菌,发廯菌和黑曲 霉均仅含有 1 个 SMC,而麦格水蚤汉氏孢虫含有 物种 Species Nosema ceranae SMC_N SMC_hinge 蛋白序列号 Protein sequence ID XP_...
YME1 like 1 ATPase 04 KEAP1 kelch like ECH associated protein 1 04 DYNC1I2 dynein cytoplasmic 1 intermediate chain 2 04 CDKN2A cyclin dependent kinase inhibitor 2A 03 HLA-B major histocompatibility complex, class I, B 03 PROTEIN PROCESSING IN THE ENDOPLASMIC RETICULUM DNAJA1 DnaJ heat shock...
To analyse the influence of condensin on extrusive cohesin, we further engineered a triple-inducible degron cell line with an additional mAID tag fused to the endogenous SMC2 protein (WapldTagSmc2mAIDCdca5mAID cells) (Fig. 1e and Extended Data Fig. 4a,g,h). Subjecting these cells and Wap...
K.Y. also performed growth measurement, RNA-seq, and ChIP-seq. N.T. performed 3C-seq on T. acidophilum and DNA affinity purification. T.T. and I.H. performed mass spectrometry analysis. K.Y. and S.I. performed protein purification to raise antibodies for ChIP-seq. K.Y., A.O., ...
The crystal structure of the hinge domain of the Escherichia coli structural maintenance of chromosomes protein MukB J. Mol. Biol., 395 (2010), pp. 11-19 View PDFView articleGoogle Scholar 90 B. Ku, J.-H. Lim, H.-C. Shin, S.-Y. Shin, B.-H. Oh Crystal structure of the MukB...
On the basis of large-scale protein engineering approaches involving a systematic change in the length of the arm and the random insertion of peptides, we have uncovered an unanticipated role that goes beyond its proposed function as a DNA barrier. The novel activity is tightly coupled to the ...
The structure of all SMC proteins resembles an elongated rod that contains a central coiled-coil domain, a common protein structural motif in which two α-helices twist together. In recent years, the imperative role of the coiled-coil domain to SMC protein activity and regulation has become ...