Analysis of this protein family confirmed that seven of its members, encoded by essential genes, are homologues of metazoan Sm proteins. Immunoprecipitation revealed that an evolutionarily related subgroup of seven Sm-like proteins is directly associated with the nuclear U6 and pre-RNase P RNAs. The...
In contrast, the Uss1 protein is a non-canonical member of the Sm-like protein family in that it associates with the U6 snRNA. A combination of molecular genetic and biochemical analysis has been used to investigate the molecular associations of the Uss1 protein. Exhaustive two-hybrid screens ...
Protein BindingAmino Acid SequenceThe bacterial Hfq protein modulates the stability or the translation of mRNAs and has recently been shown to interact with small regulatory RNAs in E. coli. Here we show that Hfq belongs to the large family of Sm and Sm-like proteins: it contains a conserved...
Protein structure prediction was performed using the AlphaFold2 program [33] based on known amino acid sequences of the protein family; an initial model was generated, followed by outputting the protein 3D structure in the PyMOL software (https://pymol.org/2/). Chromosome localization Chromosome...