SMART-Seq Human TCR (with UMIs) enables users to analyze T-cell receptor (TCR) repertoires from bulk RNA samples. This kit can generate libraries from total RNA (10 ng to 1 µg from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, or 1 ng to 10...
Panel A. 对10 ng小鼠脾脏来源 RNA使用SMART-Seq Mouse TCR (with UMIs)制备TRA和TRB文库。其中蓝线和紫线分别是指经过UMI修正误差和和没有经过UMI修正误差的数据。 Panel B. 为了评估SMART-Seq Mouse TCR (with UMIs)的再现性,使用该试剂盒分别对10 ng和100 ng小鼠脾RNA起始样本构建TCR文库。然后在Illumina ...
The SMART-Seq Mouse TCR (with UMIs) kit enables detection of mouse TCR sequences with sensitivity and no bias.
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
The relationship between stress and levels of biological substances such as cortisol, a hormone related to the human alertness, has also been studied [2,3]. We can find many definitions of the stress concept from different perspectives; sometimes, it leads to some confusion of the concept and ...