The SMART-Seq Mouse TCR (with UMIs) kit enables detection of mouse TCR sequences with sensitivity and no bias.
Panel A. 对10 ng小鼠脾脏来源 RNA使用SMART-Seq Mouse TCR (with UMIs)制备TRA和TRB文库。其中蓝线和紫线分别是指经过UMI修正误差和和没有经过UMI修正误差的数据。 Panel B. 为了评估SMART-Seq Mouse TCR (with UMIs)的再现性,使用该试剂盒分别对10 ng和100 ng小鼠脾RNA起始样本构建TCR文库。然后在Illumina ...
该文对结直肠癌T细胞进行单细胞研究,系统性地描述结直肠癌相关T细胞的组织分布特性、克隆性、迁移性和状态转化。利用T细胞受体(TCR)作为标签,研究团队发现肿瘤微环境和TCR共同影响了肿瘤浸润CD8效应记忆T细胞向耗竭性T细胞和效应T细胞的转化。 ✦应用实例2:肝癌免疫微环境 肝细胞癌是世界范围内死亡率排名第三的癌症,其...
NOTE: SMART-Seq Human TCR (with UMIs) is an equivalent replacement for the SMARTer Human TCR a/b Profiling Kit v2 with minor updates. (See a complete list of kits with new names and the existing kits they will replace here). The update (listed below) does not in any way impact the ...
b, Smartseq3xpress-based TCR reconstruction (TRaCeR) overlayed onto UMAP. c, QC of TCR reconstructions obtained with Scirpy, enumerating the number of T cells with certain types of TCR reconstructions. d, Benchmarking of Smart-seq2, Smart-seq3 and Smart-seq3xpress (SeqAmp, improved TSO) ...
1技术简介单细胞转录组测序(Single Cell RNA Sequencing)是在单细胞水平对mRNA进行扩增及高通量测序的一种技术,主要研究单个细胞内的整体基因表达情况,以及基因的结构变异;广泛用于细胞分子机制中细胞异质性研究、以及样本量少而无法进行常规高通量测序等。2 技
Single-cell TCR sequencingWe developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture ...
invivoscribe代理商之产品12000011-T淋 巴细胞基因重排检测试剂盒 TCRB + TCRG T-Cell Clonality Assay ABI Fluorescence invivoscribe代理商之产品11000031-B淋 巴细胞基因重排检测试剂盒 IGH + IGK B-Cell Clonality Assay ABI Fluorescence 产品目录 •Spirochrome •VMRD •broadpharm •细胞因子 •胎...
Detailed quantitative analysis of GFP expression in SAHA and TCR-treated cells. Cells were prepared for single cell analysis at the Genome Technology Facility (GTF) of the University of Lausanne. Cells were loaded on Fluidigm C1 IFC plat... M. Golumbeanu,S. Rato,S. Cristinelli,... - 《...
该文对结直肠癌T细胞进行单细胞研究,系统性地描述结直肠癌相关T细胞的组织分布特性、克隆性、迁移性和状态转化。利用T细胞受体(TCR)作为标签,研究团队发现肿瘤微环境和TCR共同影响了肿瘤浸润CD8效应记忆T细胞向耗竭性T细胞和效应T细胞的转化。 ✦应用实例2:肝癌免疫微环境 ...