Several functional domains in the FBX proteins, including kelch repeats, leucine-rich repeats, WD40 repeats, F-box associated (FBA) domain, tubby domain, phloem protein 2 and an unknown functional domain were identified in silico. We classified the FBX-coding genes into 8 subfamilies based on ...
In addition, eight zinc finger transcription factors and several transcription factors of other types (Table 4) also ex- hibited elevated protein levels in ask1, indicating that destabilization of these transcription factors by ASK1-E3s might be important for floral development. The ask1 mutation ...
MD simulation was carried on the modelled structure of the protein (VrSKP1) for 180 ns. Gromacs 5.1.4 software package49 has been used with the OPLS-AA force-field. The whole system was then solvated using simple point charge (SPC) water model in a cubic box and 16 Na+ counter ions ...
Theask1mutation also caused an increased level of a Polycomb group protein, SWINGER (SWN) (Table4), which interacts with other Polycomb group proteins to repress expression ofFLOWERING LOCUS C(FLC) and controls the initiation of endosperm development [55–57]. The elevated SWN protein level is ...
The ARABIDOPSIS SKP1-LIKE1 (ASK1) protein functions as a subunit of SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases. Previous genetic studies showed that ASK1 plays important roles in Arabidopsis flower development and male meiosis. However, the molecular imp
It is widely accepted that an F-box protein directs substrate ubiquitination within a Skp1·CUL1·F-box protein·ROC1 (SCF-ROC1) E3 ubiquitin ligase complex. This assembly utilizes the CUL1 molecular scaffold, allowing the F-box protein to position its bound substrate for ubiquitination by a ...
Protein degradation and turnover under various environmental stresses is basically regulated by ubiquitin-proteasome system (UPS), of which SKP1 is a very essential component. Isolation and cloning of an identified potential stress responsive candidate gene SKP1, was successfully done for the first ...