龙眼SKP1-like家族全基因组鉴定分子特性体胚发生早期表达模式为研究S-phase kinase-associated protein 1-like(SKP1-like)在龙眼中的分子特性以及其在体胚发生早期的表达模式,基于模式植物拟南芥SKP1-like的氨基酸序列,在龙眼基因组数据库中进行SKP1-like成员的鉴定.同时,通过生物信息学方法对其蛋白理化性质,系统进化特...
The F-box protein UNUSUAL FLORAL ORGANS (UFO) is important for normal meristem identity and floral organ development [17–19]. UFO can interact with LEAFY genetically to activate AP3 expression [20–22]. The Arabidopsis homolog of the yeast and human SKP1 genes, the ARABIDOPSIS SKP1-LIKE1 ...
As a core subunit of the SCF complex that promotes protein degradation through the 26S proteasome, S-phase kinase-associated protein 1 (SKP1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (GA), jasmonate, ethylene, auxin and light responses. P7-2 encode...
Most of the known ubiquitin ligase substrates were identified by protein-protein interaction methods, usually when the F-box protein has a known function [10,32–34]. Recently, mass spectrometry (MS)-based proteomics approaches have been increasingly applied in various areas including differential gen...
The ARABIDOPSIS SKP1-LIKE1 (ASK1) protein functions as a subunit of SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases. Previous genetic studies showed that ASK1 plays important roles in Arabidopsis flower development and male meiosis. However, the molecular impact of ASK1-containing SCF E3 ubiquitin...
Protein degradation and turnover under various environmental stresses is basically regulated by ubiquitin-proteasome system (UPS), of which SKP1 is a very essential component. Isolation and cloning of an identified potential stress responsive candidate gene SKP1, was successfully done for the first tim...
expression analysisUbiquitination participates in plant hormone signaling and stress response to adversity. SKP1-Like, a core component of the SCF (Skp1-Cullin-F-box) complex, is the final step in catalyzing the ubiquitin-mediated protein degradation pathway. However, the SKP1-Li...
Expression analysisSub-cellular localizationYeast two-hybridizationSKP1 is a core component of SCF complex, a major type of E3 ubiquitin ligase catalyzing the last step in ubiquitin-mediated protein degradation pathway. In present study, SKP1 gene family in Solanum pimpinellifolium (SSK), a wild ...