网络定点整合;位点特异整合;整合的定向性 网络释义
位点特异性整合 Site-specific integrationThe present invention relates to stable and high-producing site- specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.J・朗斯...
Efficient Site-Specific Integration of Large Transgenes by an Enhanced Herpes Simplex Virus/Adeno-Associated Virus Hybrid Amplicon Vector We previously demonstrated that a herpes simplex virus type 1 (HSV-1)/adeno-associated virus (AAV) hybrid amplicon vector constructed by inserting the sequ... Q ...
OPEN SUBJECT AREAS: MOLECULAR BIOLOGY BIOTECHNOLOGY GENETIC ENGINEERING Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway Jae Seong Lee, Thomas Beuchert Kallehauge, Lasse Ebdrup Pedersen & Helene Faustrup Kildegaard Received 20 October 2014 Accepted ...
HONG KONG, Oct. 11, 2022 /PRNewswire/ -- Great Bay Bio Announces Official Launch of Site-Specific Integration Platform for Cell Line Development, AlfaCell®
The recombinant prfC allele created upon element integration is functional. The integration and excision mechanism of the SXT element shares many features with site-specific recombination found in lambdoid phages. First, like λ, the SXT element forms a 展开 ...
The DNA sequence motifs which direct adeno-associated virus type 2 site-specific integration are being investigated using a shuttle vector, propagated as a... P Meneses,KI Berns,E Winocour - 《Journal of Virology》 被引量: 149发表: 2000年 Herpes Simplex Virus Type 1/Adeno-Associated Virus Hyb...
专利名称：SIMULTANEOUS SITE-SPECIFIC INTEGRATIONS OF MULTIPLE GENE-COPIES IN FILAMENTOUS FUNGI 发明人：UDAGAWA, Hiroaki 申请号：EP12723652.9 申请日：20120523 公开号：EP2714914A1 公开日：20140409 专利内容由知识产权出版社提供 摘要：The invention relates to a method for the simultanoues integration of ...
Here, we aimed to achieve site-specific integration of FSI-I-I, including the signal peptide, N terminus, and three FSI domains, into the last codon of the porcine MSTN gene using a homology-mediated end joining (HMEJ)-based strategy mediated by CRISPR-Cas9. Based on somatic cell nuclear ...
An in vitro system to study the mechanism of site-specific integration of adeno-associated virus (AAV) was developed. This system is based on two substrates, a linear or circular AAV donor and a circular acceptor containing the preintegration locus AAVS1. In the presence of HeLa extract and ...