In this study, we present a highly efficient site-specific integration strategy based on homologous recombination and 桅C31 integrase. An attB sequence was introduced at the homologous arm of an insertion targeting vector. \\{DSBs\\} at the target locus and donor were then simultaneously generated...
In this paper, we present the Nexus approach to efficient domain-specific integration of many loosely coupled data sources. A so called information maximizing mediation middleware (IMMM) has to cope with large data volumes and many queries, and at the same time achieve a tight semantic integration...
Large serine recombinases (LSRs) are DNA integrases that facilitate the site-specific integration of mobile genetic elements into bacterial genomes. Only a few LSRs, such as Bxb1 and PhiC31, have been characterized to date, with limited efficiency as tools for DNA integration in human cells. ...
Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J. 7, 649–659 (1995). Article CAS PubMed Google Scholar Horii, T. et al. Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering. ...
If we talk about the integration of modern technology in the world of trading and money monitoring, there aren’t many trading platforms and applications that can track your stock growth and other related trends through the power of AI. But Trade Ideas is one of the very few such platforms ...
Microinjection of Cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient ... TD Wit,D Drabek,F Grosveld - 《...
The introduction of engineered site-specific DNA endonucleases has brought precise genome editing in many model organisms and human cells into the realm of... U Irion,J Krauss,C Nusslein-Volhard - 《Development》 被引量: 202发表: 2014年 Efficient CRISPR-Cas9-mediated genome editing in Plasmodium...
In this specific scenario, it is evident that the outcomes derived from the master equation approach may overestimate the magnitude when compared to the HEOM results in the high-frequency range of the PSD. The origin of this inconsistency can be attributed to the non-Markovian effect5,9,81,...
F. Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin. Proc. Natl. Acad. Sci. U S A 88, 3111–3115 (1991). Article CAS ADS PubMed PubMed Central Google Scholar Tao, P., Wu, X...
Despite having access to antibody-specific sequence variation, both the AbLang and Sapiens models also consistently underperformed the general protein language models and even underperformed the site-independent models when recommending substitutions to mAb114 UCA (Supplementary Table 12 and Supplementary ...