Stoll SM, Ginsburg DS, Calos MP (2002) Phage TP901-1 site-specific integrase functions in human cells. J Bacteriol 184(13):3657–3663 CentralStoll, S.M. et al. (2002) Phage TP901-1 site-specific integrase functions in human cells. J. Bacteriol. 184, 3657-3663...
814. Integration Sites Used by the PhiC31 Site-Specific Integrase in the Human GenomeRNA transfectiondendritic cellcytotoxic T lymphocytesadoptive therapyvaccine therapymt is a cross-disciplinary biomedical journal devoted to publishing the most exciting advances in pharmacology and therapeutics, as they ...
In vivo site-specific integration of transgene in silkworm via PhiC31 integrase-mediated cassette exchange Invivo recombinase-mediated cassette exchange (RMCE) was first achieved in Bombyx mori.Frequencies of RMCE events are different when the incoming att sites... D Long,A Zhao,L Xu,... - 《...
(2006). Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase. Nat. Methods 3, 615-621.Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, et al. (2006) Efficient site-specific integration in Plasmodium falciparum chromosomes ...
aureofaciens encodes a putative integrase, a basic protein of 416 amino acids. The μ1/6 integrase was found to belong to the integrase family of site-specific tyrosine recombinases. The phage attachment site () was localized downstream of the gene. The attachment junctions ( and ) were determ...
2016: Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase Methods in Molecular Biology 1469: 15-30 Sun JiaQiang; Zuo JianRu 2003: Excision of selectable marker genes from transgenic plants by site-specific DNA recombination AgBiotechNet 5(ABN 107): 1-4 Hong-...
and Ow, D.W. (2016) Method for biolistic site-specific integration in rice and tobacco catalyzed by Bxb1 integrase. In: Methods Mol Biol: Chromosome and Genomic Engineering in Plants, vol. 1469 (Murata, M., ed), pp. 15-30. New York: Humana Press....
Further, scIHF2 is required for site-specific integrative recombination by phage lambda integrase and for pSC101 replication in a DeltaIHF E.coli host. It also triggers site-specific integrative and excisive recombination in vitro to the same extent as the wild-type protein. We also demonstrate...
The method of inserting a polynucleotide sequence into a genome of a human cell may include: introducing into the human cell a circular nucleic acid comprising the polynucleotide sequence flanked by a phiC31 first recombination site and a Bxb1 first recombination site; a phiC31 integrase; and a...
Recombination occurs within specific phage and bacterial attachment sites and is catalyzed by the phage-encoded integrase protein in vivo. We describe here the overexpression and purification of L5 integrase and its ability to mediate integrative recombination in vitro. We find that L5 integrase-...