siRNA deep sequencing and assembly: piecing together viral infections. In: Gullino, M.L., Bonants, P.J.M. (Eds.), Detection and Diagnostics of Plant Pathogens. Springer, Dordrecht, 21-38.Kreuze J. siRNA Deep Sequencing and Assembly: Piecing Together Viral Infections. In: Detection and ...
Mbatchou, J., Locke, A. E., Benner, C., Verweij, N., Lin, N., Hossain, S., Agostinucci, K., Pascale, J. V., Dirice, E., Dunn, M., … Lotta, L. A. (2021). Sequencing of 640,000 exomes identifies GPR
"jetPRIME gave better transfection efficiencies than other reagents tested and much less cell death was noticed. The cells don't seem to mind being treated with the transfection reagent, there is no need to change the media anyway. I've already ordered a bigger volume of jetPRIME and have use...
原理一是non-sequencing,即直接在库中利用sequence找相似miRNA的片段,根据该片段序列对应的结构信息,推测该miRScan的结构,从而得到该未知miRNA的茎环结构、茎环范围、五连体、凸起和额外pair sequence的信息。 原理二是sequencing,即因为NGS的推广而出现的miRNA-seq,通过bioinformation来得到其表达谱,identify存在的miRNA或预...
siRNA is a simple and efficient tool to modulate gene expression. So siRNA is widely used in target identification and validation, especially Large-scale siRNA screen present high efficiency and relative lower cost. Hitgen has the capability of siRNA design, synthesis, and cell phenotype characterizat...
此外,华大智造将撤销在加州北部地区法院对因美纳的反垄断诉讼。因美纳将获得华大智造及其子公司CG的“双色测序技术(Two-color sequencing technology)”系列专利授权。如同此前所宣布的,华大智造将于2022年8月开始在美国销售其基于CoolMPS技术的相关测序产品,并于20...
As the inserted fragments in the constructed siRNA expression vector can form a hairpin structure, this may affect sequencing. PCR products obtained from plasmid amplification will be sequenced if we have unsatisfactory plasmid sequencing results. ...
Sequencing of the emerged RNAi-resistant virus showed that thenefsequence was altered in several ways: in some cases the siRNA recognition site contained several nucleotide substitutions which disrupted base-pairing and in other cases the target was simply deleted. Similar mutation patterns were also ...
The Human Genome Project as well as substantial progress in technologies such as next-generation sequencing and other major breakthroughs in the last decades have provided a wealth of information about the human genome and the pathophysiological role of genetic factors in various diseases [153,154]....
Selecting aptamers that bind specifically to target cells or tissues is a crucial step in developing an aptamer-mediated siRNA delivery system. Recent developments in aptamer selection technologies, such as SELEX and high-throughput sequencing, have enabled the identification of aptamers with high affinit...